两种稀释剂在自制乙型肝炎五项室内质控品中的应用效果

The application effect analysis of two kinds of thinners in homemade hepatitis B five internal quality control products

目的通过比较两种稀释剂在自制乙型肝炎五项室内质控品中的应用效果，再结合两种稀释剂的各自特点，确定自制乙型肝炎五项室内质控品时的最佳稀释剂。方法将乙型肝炎五项阳性血清分别以10％小牛血清磷酸盐缓冲液和0.85％NaCl溶液进行稀释，自制室内质控品，对用两种稀释剂自制的质控品同时以ELISA法进行检测，对检测结果进行分析。结果以两种稀释剂制备的乙型肝炎五项质控品，其各项检测结果比较，差异无统计学意义（P〉0.05）。结论虽然10％小牛血清磷酸盐缓冲液和0.85％NaCl溶液在自制乙型肝炎五项室内质控品中的应用效果差异无统计学意义，但0.85％NaCl因其不合异嗜性抗原、成本低廉，为自制乙型肝炎五项室内质控品的最佳稀释剂。

Objective By comparing the application effect of two diluents in homemade hepatitis B five internal quality control products, and then combining the characteristics of two diluents, they were determined the best thinner of homemade hepatitis five indoor quality control materials. Methods The positive serum of hepatitis B five were re- spectively diluted with 10% fetal calf serum phosphate buffer and 0.85% NaC1 solution,and homemade indoor quali- ty control materials, ELISA detected homemade quality control materials of two kind of thinners, the results were an- alyzed. Results Comparison of the test results of hepatitis B five quality control materials of two kinds of diluents was no significant difference（P〉0. 05）. Conclusion Although there was no statistically significant difference be- tween 10% fetal calf serum phosphate buffer and 0.85% NaC1 solution in homemade hepatitis B five internal quality control products,0.85 % NaC1 was made the best thinner of homemade hepatitis five indoor quality control materials because it did not contain pica antigen, low cost.