碳纳米管/聚吡咯导电水凝胶修饰生物电极的研究

Research on carbon nanotubes/polypyrrole conductive hydrogel modified bioelectrode

以水凝胶钛网电极为基底的生物电极,通过在水凝胶中添加聚吡咯(PPy)、碳纳米管(SWNTs)和神经生长因子(NGF)形成不同的杂化水凝胶修饰电极,研究不同杂化水凝胶修饰生物电极的力学性能、电化学活性以及对细胞分化生长情况的影响。力学测试表明SWNTs和PPy能够显著提高水凝胶的压缩模量;循环伏安(CV)曲线表明SWNTs和PPy能提高杂化水凝胶修饰电极的电化学活性;细胞形貌观察发现NGF能够促进细胞分化,SWNTs对细胞分化有增强作用,PPy可以提高细胞的黏附并有利于释放NGF,以促进细胞的生长与分化。

Titanium mesh electrode was modified with hybrid hydrogels composed of a conducting polymer of polypyrrole (PPy), single-walled carbon nanotubes (SWNTs),nerve growth factor (NGF)and a polymeric hydrogel of poly(ethylene glycol)diacry-late-polyacrylamide (PEGDA-PAAm).Mechanical properties and electrochemical performance as well as its effect on neuron growth were investigated.The results show that the incorporation of SWNTs and PPy dramatically enhances the compression modulus of the hydrogel.Meanwhile,the incorporated SWNTs and PPy greatly improve the electrochemical performance of the hybrid hydrogels,demonstrated by the cyclic voltammetry (CV)measurements.Observation of cell morphology indicates that NGF can enable the differentiation of the cells while SWNTs can help the enhancement.More importantly,PPy is favorable for neuron attachment and the release of NGF,which in turn promotes the growth and differentiation of the neural cells.