金刚藤口服液基于免疫调节对大鼠慢性盆腔炎的治疗作用研究

Study of the Therapeutic Effect on Rats Chronic Pelvic Inflammatory Disease of Bullbrier Polysaccharide

目的:从免疫调节角度研究金刚藤口服液对大鼠慢性盆腔炎的治疗作用。方法:选择未交配的SPF级雌性SD大鼠60只,随机分为空白组、模型组、阳性(金刚藤胶囊)组、金刚藤口服液低、中、高剂量组。实验第1天,除空白组外,其余各组大鼠用戊巴比妥麻醉,采用双侧输卵管注入30%苯酚胶浆生理盐水溶液,制作大鼠盆腔炎模型。造模后正常组和模型组ig给予纯净水,其余各组ig给予药物,其中阳性组日给药量为540 mg·kg-1,金刚藤口服液低、中、高剂量组日给药量分别为:15 mg·kg-1、30 mg·kg-1、60 mg·kg-1,每天1次,共ig治疗20天。第21天断颈处死动物,ELISA法测定各实验组大鼠血浆IgA、IgM和IgG含量;剖取子宫,称重后做病理形态分析。结果:与正常组比较,模型组大鼠子宫重量系数[(11.7±1.92)mg·g-1]极显著增大(P<0.01),血浆IgA[(27.99±5.49)μg·mL-1]、IgM[(21.94±1.98)μg·mL-1]和IgG[(4.85±1.97)μg·mL-1]含量均有极显著减少(P<0.01);子宫炎症性病变显著加重;与模型组比较,金刚藤口服液组可显著减小大鼠子宫重量系数(P<0.05),增加血浆IgA、IgM和IgG含量(P<0.05),且能减轻子宫炎症性病变。结论:金刚藤口服液可通过调节大鼠血浆免疫球蛋白浓度而对慢性盆腔炎产生治疗作用。

Objective： To study the therapeutic effect on rat chronic pelvic inflammatory disease of bullbrier polysaccharide. Methods： unmated SPF 60 female SD rats were randomly divided into control group, model group, positive group （Bullbrier capsules） group, bullbrier polysaccharide low, medium and high dose groups. On the 1d of experiment, except the blank group, the rest of the rats were anesthetized with amyl barbiturate, phenol 30% slurry injection of saline solution was injected from bilateral tubal to make rats pelvic inflammatory disease model. After being modeled, the normal group and model group ig given pure water, the positive group were given bullbrier dose of 540 mg·kg-1, polysaccharide of bullbrier of low, medium and high dose groups were given of dose： 15 mg·kg-1, 30 mg·kg-1, 60 mg·kg-1, a total ig 20 days of treatment. On the 21th days, rats were sacrificed, Elisa method was used for the determination of the experimental rats plasma IgA, IgM and IgG levels; taken uterus, after being weighed, pathological mor-phological analysis were done. Results： Compared with the normal group, model group uterine weight coef-ficient [（11.7±1.92） mg·g-1] was significantly increased （P〈0.01）, plasma IgA [（27.99±5.49） μg·mL -1], IgM [（21.94±1.98） μg·mL-1] and IgG[（4.85±1.97） μg·mL-1] content are extremely significantly reduce（P〈0.01）; the uterine inflammatory lesions were significantly heavier; compared with model group, Smilax polysaccharides can significantly reduce the weight of the rat uterus coefficient（P〈0.05）, increased plasma IgA, IgM and IgG levels （P〈0.05）, can reduce the uterine inflammatory lesions. Conclusion： Bullbrier polysaccharide can treat chronic pelvic inflammatory disease by adjusting the concentration of rat plasma immunoglobulin.