摘要
建立了17种兽药中非法添加氨茶碱和二羟丙茶碱的高效液相色谱法。以十八烷基键合硅胶为填充剂，0．025mol／L磷酸二氢钾溶液-甲醇为流动相，二极管阵列检测器全波长（200～400nm）扫描，270nm进行测定，并采用峰纯度检查和光谱相似度检查辅助对照品比对方法，对非法添加药物进行确证。在此液相色谱条件下，氨茶碱和二羟丙茶碱与其他物质峰能很好的分离。两种药物在0．5～200μg／mL范围内线性关系良好，相关系数r均为1．0000。按外标法以峰面积计算，氨茶碱和二羟丙茶碱的平均回收率分别为92．6％～108．3％、93．5％-105．9％，RSD分别为0．05％～3．6％、0．2％～3．7％。结果表明，该方法适合兽药中氨茶碱和二羟丙茶碱的检测。
A method for the determination of aminophylline and diprophylline in 17 kinds of veterinary drugs was developed by the high performance liquid chromatography with photo-diode array detector （HPLC-PDA）. It was tested with C18 column, using 0.025 mol/L potassium dihydrogen phosphate solution and methanol as the mobile phase. The detection wavelength was set at 270 nm and simultaneously collected the spectrum at wavelength of 200-400 nm. Peak purity test and spectrum similar test were helped to identify the aminophylline and diprophylline. The concentration of the aminophylline and diprophylline in the range of 0.5- 200 μg/mL showed a good linear relationship, r= 1.0000. The average recoveries of the drugs ranged from 92.6% to 108.3% at spiked levels with RSD less than 3.7%. The results showed that the method was suitable for detection of aminophylline and diprophylline in veterinary drugs.
出处
《中国兽药杂志》
2014年第12期32-38,共7页
Chinese Journal of Veterinary Drug
基金
河南省重点科技攻关计划(编号:132102110078)
