摘要
建立了高效液相色谱法测定五种制剂中非法添加喹乙醇与乙酰甲喹的方法。以十八烷基键合硅胶为填充剂，乙腈－0．01 mol／L磷酸二氢钾（ pH 6．0）为流动相，二极管阵列检测器进行全波长（200～400 nm）扫描，检测波长为260 nm，并采用峰纯度检查和光谱相似度检查辅助对照品比对方法，对非法添加药物进行确证。结果表明，该色谱条件下喹乙醇、乙酰甲喹与其他物质峰分离良好。喹乙醇、乙酰甲喹在0．5～200μg／mL浓度范围内线性良好，回收率在91．1％～105．7％之间， RSD≤3．1％，喹乙醇、乙酰甲喹的检测限分别为1．0和2．5 mg／g。
A method of illegally adding olaquindox and mequindox in the five kinds of preparations which using the high performance liquid chromatography was studied. It was tested with C18 column, using isocratic elution with 0.01mol/L potassium dihydrogen phosphate （ pH 6. 0） and acetonitrile as the mobile phase, diode array detector scanning 200-400 nm, the detection wavelength was 260 nm. Peak purity test and spectrum similar test were helped to identify the olaquindox and mequindox. The results showed that under the chromatographic condition, olaquindox, mequindox and other materials separated well. The linearity of olaquindox, mequindox in the range of 0.5-200.0 μg/mL were good, the mean recovery of olaquindox, mequindox were 91.1%-105.7%, RSD≤3.1%, the detection limits of olaquindox and mequindox were 1.0 and 2.5 mg/g.
出处
《中国兽药杂志》
2014年第12期43-49,共7页
Chinese Journal of Veterinary Drug
基金
河南省重点科技攻关计划(编号:132102110078)
