人脑不同发育期vimentin阳性细胞的分布

Morphology and distribution of vimentin positive cells in human fetal brain at deferent gestation week

目的 研究不同发育时期人脑 vimentin阳性细胞的形态特征及其分布情况 .方法 计划生育流产或引产的胚胎(9,13及 2 8wk)大脑经多聚甲醛固定 ,石蜡包埋并连续切片 ,免疫组织化学染色 ,光镜下观察 .结果 免疫组织化学染色结果发现 vimentin阳性细胞形态多样 ,大小不一 ,多呈圆形 ,分布广泛 ,特别是嗅球、海马、纹状体以及脑室下区均可见到 vim entin阳性的细胞 ,并且随发育时期及部位不同 ,细胞形态及突起情况各异 .结论 vimentin阳性细胞形态及分布不同可能与发育阶段有关 .

AIM To observe the morphology and distribution of vimentin positive cells in human fetal brain in different gestation weeks. METHODS Fetal brain (9, 13 and 28 weeks) were fixed with formaldyhydum polymerisatum and embeded with paraffin wax. Then, the morphology and distribution of the vimentin positive cells were observed after immunohistochemical staining. RESULTS Vimentin positive cells were distributed extensively and could be foud in all the regions of the fetal brain, especially in olfactory bulb, hippocampus, striatum and subventricular zone. The shapes and sizes of vimentin positive cells were different according to the different gestation weeks and different regions of the brain. Most of them were round with or without processes. CONCLUSION Different shapes and sizes of vimentin positive cells may be related to the special development stages.