兔膝关节骨关节炎模型建立的探讨

Investigation on the Establishment of Rabbit Knee Osteoarthritis Model

目的探讨应用前交叉韧带和内外侧半月板切除的方法制作骨关节炎(OA)动物模型的可行性及可靠性。方法成年雄性新西兰大白兔20只,于右后肢膝关节切断前交叉韧带和切除内外侧半月板,左后肢仅打开关节囊,不对关节内部进行操作,观察术后兔子活动情况;于第2、6、10周处死,观察其左、右两侧后肢膝关节软骨病理改变。实验动物2、6、10周后取右后肢膝关节软骨为实验组,左侧肢体膝关节软骨标本为对照组,比较两组软骨病变情况。结果 2、6、10周后,从实验动物活动表现和大体标本观察,兔右后肢膝关节均呈典型的OA特征。结论前交叉韧带和内外侧半月板切除是一个制作OA动物模型的较为理想的方法。

Objective To explore the feasibility and reliability of application of anterior cruciate ligament and the medial and lateral meniscus resection of making animal model of OA. Method 20 adult male New Zealand rabbits ,on the right leg knee cut off the anterior erueiate ligament and excision of medial and lateral meniscus,just open the left hind limb joint capsule,no joint operation,observation of postoperative rabbit activities ; In 2, 6, 10 weeks later, observation the left, right posterior articular cartilage pathology. After 2, 6, 10 weeks, the right leg knee cartilage as the experimental group, the left limb knee joint cartilage were as control group, comparison of two groups of cartilage lesions. Result 2, 6, 10 weeks later, from the experimental animal activity and gross specimen observation, rabbit right leg knee showed the typical characteristics of OA. Conclusion anterior cruciate ligament and the medial and lateral meniscus resection is an ideal method for a OA animal model making.