纳络酮对脑创伤致急性肺损伤中对内皮素的影响

Effects of naloxone on plasma and lung tissue endothelin-1 of rat with acute lung injury caused by brain trauma

目的 研究纳络酮治疗脑创伤致急性肺损伤大鼠中对内皮素的影响 .方法 成年 Wistar大鼠随机分为对照组 (n= 10 )、脑创伤组 (n=4 0 )和纳络酮治疗组 (n=10 ) .采用Feeney脑创伤模型 ,观察脑创伤后大鼠不同时间点肺组织的病理改变 ,放免法测定血浆和肺组织 ET- 1含量变化及应用纳络酮对其的影响 .结果 脑创伤大鼠出现程度不同的急性肺损伤的病理改变 ,尤以伤后 6 h为重 .血浆和肺组织 ET- 1含量明显增高 (2 0 1.7± 6 3.7vs 2 1.8± 1.9,P<0 .0 1;190 .2± 6 5 .6 vs 17.5± 0 .8,P<0 .0 1) ,伤后 6 h达峰值 ,72 h仍持续性升高 .治疗组肺组织病理改变明显减轻 ,血浆和肺组织ET- 1尽管于伤后 72 h仍高于对照组 (30 .4± 3.1vs2 1.8±1.9,P<0 .0 1;2 5 .6± 1.8vs17.5± 0 .8,P<0 .0 1) ,但明显低于创伤组 (30 .4± 3.1vs 96 .4± 18.8,P<0 .0 1;2 5 .6± 1.8vs 6 0 .4± 19.0 ,P<0 .0 1) .结论 脑创伤可致程度不同的急性肺损伤 ,ET- 1可能在其发病过程中起重要作用 ,纳络酮对急性肺损伤有一定的治疗作用 .

AIM Effect of naloxone on content of endothelin 1(ET 1) in the plasma and lung tissue of rat with acute lung injury caused by brain trauma and pathological changes were observed. METHODS Wistar rats were randomly divided into control (Ctr n =10), brain trauma (BT n =40) and naloxone groups(Nal n =10). Pathologic changes of lung in model of brain trauma of rats were observed. The levels of ET 1 in the plasma and lung tissue were determined by radioimmunassay. RESULTS Brain trauma can cause some degrees acute lung injury in special at 6h after injury. The levels of ET 1 in the plasma and lung tissues increased significantly after brain trauma (201.7±63.7 vs 21.8±1.9, P <0.01;190.2±65.6 vs 17.5±0.8, P <0.01), arriving the highest level at 6 h, but still higher at 72 h. Pathologic changes of rat with naloxone treating were abated markedly. The levels of ET 1 in the plasma and lung tissue of rat with naloxone treating were higher than control group (30.4±3.1 vs 21.8±1.9, P <0.01;25.6±1.8 vs 17.5±0.8, P < 0.01), but significantly lower than trauma groups (30.4± 3.1 vs 96.4±18.8, P <0.01;25.6±1.8 vs 60.4±19.0, P < 0.01). CONCLUSION Brain trauma can caused some degrees acute lung injury; ET 1 may play an important role in pathophsiology of acute lung injury caused by brain trauma; naloxone can abate its injury effects.