祖师麻药材HPLC特征图谱

Characteristic Spectrum of Zushima by HPLC

采用高效液相色谱法,建立了祖师麻药材的HPLC特征图谱。色谱柱为C18(5μm:4.6 mm×250 mm),以乙腈-0.05%磷酸溶液(9∶91)为流动相,流速0.9 m L/min,检测波长326 nm。特征图谱中有3个特征峰,以参照物祖师麻甲素的峰为S峰,其保留时间定为1,其他特征峰相对保留时间规定为:0.46(峰1)、0.79(峰2)、1.00(峰S)。经考察,所建立的祖师麻药材特征图谱耐用性较好,能很好的控制祖师麻药材的质量。

The characteristic spectrum of Zushima was extablished by High Performance Liquid Chromatography（ HPLC）. The method of High Performance Liquid Chromatography was carried out on a column of C18,using the mobile phase of Acetonitrile – 0. 05% Phosphate（ 9 ∶ 91） with a flow rate of 0. 9 m L / min. The detection wavelength was 326 nm. There were three characteristic peaks in characteristic spectra. Daphnetin＇s peak was reference and the retention time was one,other chaacteristic peaks relative retention time were： 0. 46（ peak 1）,0. 79（ peak 2）,1. 00（ peak S）. After inspection,the method had good durability,and could well to control the quality of Zushima.