咳喘停贴剂穴位贴敷对慢性哮喘气道炎症大鼠肺组织T-bet、GATA-3 mRNA表达的影响

Effect of Acupiont Sticking Therapy with Kechuanting Paste on Expression of T-bet and GATA-3 mRNA in Lung for Chronic Asthma Airway Inflammation Rats

目的观察咳喘停贴剂穴位贴敷治疗对慢性哮喘气道炎症大鼠以Th1/Th2为主的上游特异性转录因子表达的影响。方法将50只Wistar雄性大鼠随机分成5组,每组10只,分别为正常组(A)、哮喘模型组(B)、地塞米松组(C)、假穴位贴敷组(D)、穴位贴敷组(E)。B、C、D、E组以卵蛋白(OVA)致敏和激发制作慢性哮喘气道炎症模型,观察治疗后各组大鼠肺组织Th1/Th2上游特异转录因子T-bet、GATA-3mRNA表达的差异性。结果与正常组比较,哮喘模型组、假穴位贴敷组T-bet mRNA含量降低,GATA-3mRNA含量升高,T-bet/GATA-3比值下降(P<0.05);地塞米松组、穴位贴敷组则差异无统计学意义。与哮喘模型组比较,地塞米松组、穴位贴敷组T-bet mRNA均升高,GATA-3mRNA均降低,T-bet/GATA-3比值均升高(P<0.05);假穴位贴敷组则差异无统计学意义。地塞米松组、穴位贴敷组之间差异无统计学意义。结论咳喘停贴剂穴位贴敷治疗慢性哮喘气道炎症,可通过增加Th1相关T-bet mRNA的表达,降低Th2相关GATA-3 mRNA的表达来调节Th1/Th2的失衡,从而起到改善慢性气道炎症的作用。咳喘停贴剂穴位贴敷治疗的疗效与地塞米松相似。

OBJECTIVE To observe effect of acupoint sticking therapy with Kechuanting paste on expression of Th1/Th2-dominated transcription factor in rats with chronic asthma airway inflammation.METHODS 50 male Wistar rats were randomly divided into five groups on average,including control group （A）,asthma model group （B）,dexamethasone group （C）,sham acupoint sticking therapy group （D） and acupoint sticking therapy group （E）.Rats in groups B,C,D and E were sensitized and stimulated with ovalbumin （OVA） for asthma models,which were evaluated through rat behavior and pulmonary pathological changes.The differences of T-bet and GATA-3 mRNA expression were observed.RESULTS Compared with A,B and D showed less T-bet mRNA,more GATA-3 mRNA and lower T-bet/GATA-3（P〈0.05） while there was no significant difference in C and E.Compared with B,C and E showed higher T-bet mRNA,lower GATA-3 mRNA and higher T-bet/GATA-3（P〈0.05） while there was no significant difference in D.There was no significant difference in C and E for T-bet mRNA,GATA-3 mRNA and T-bet/GATA-3.CONCLUSION Ke Chuanting acupoint sticking therapy can improve symptoms of chronic asthma airway inflammation by increasing expression of Th1-related T-bet mRNA and decreasing expression of Th2-related GATA-3 mRNA to regulate the imbalance of Th1/Th2,thus improving chronic airway inflammation.The effect of acupoint sticking therapy with Kechuanting paste may be similar to that of dexamethasone.