覆瓦蓟根部挥发油成份及抗氧化活性的初步研究

A Preliminary Study on Chemical Constituents and Antioxidant Activity of the Volatile Oil from the Root of Cirsium leducei( Franch. ) Levl.

为了研究覆瓦蓟Cirsium leducei(Franch.)Levl.根部挥发油化学成分及其抗氧化活性,采用水蒸气蒸馏法,从覆瓦蓟根部提取挥发油,结合GC-MS联用技术进行化学成分分析,利用铁氰化钾还原法和水杨酸法初步探讨其抗氧化活性。结果表明,从覆瓦蓟根部挥发油中共分离出13个化合物,鉴定出其中11个成分,随着浓度增加,覆瓦蓟根部挥发油的还原能力和对OH·自由基的清除能力逐渐增强。结果揭示,覆瓦蓟根部挥发油主要成分为脂肪烃类和萜类及其衍生物,具有一定的抗氧化能力。

To analyze the chemical constituents and the antioxidant activity of the volatile oil from the root of Cirsium leducei （Franch.） Levi. , the essential oil were extracted by steam distillation and analyzed by gas chromatography- mass spectrometry. The antioxidant activity was measured by potassium ferricyanide reduction method and salicylic acid assay, respectively . Thirteen compounds were separated and eleven of them were identified. The reducing capacity and scavenging capacity against OH ·enhanced with the increase of the concentration of the volatile oil. The results indicated that the main components of the essen- tial oil were aliphatics, terpenes and their derivatives. The volatile oil has potential antioxidation ability.