胰岛素样生长因子Ⅱ及其受体在肝组织中的表达意义

Expression and significance of insulin-like growth factor Ⅱ and its receptor in liver tissue

目的 研究胰岛素样生长因子 (IGF- )及其受体(IGFR)在不同肝组织中的表达及与肝癌病理学分级及乙肝病毒感染的关系 .方法 采用免疫组织化学方法 ,检测 10例正常肝标本、2 8例肝血管瘤标本、13例肝硬化标本和 4 2例原发性肝癌中的 IGF- 及 IGFR的表达水平 .分析其阳性率及与乙型肝炎病毒 (HBV)感染和 Edmondson分级的关系 .结果 正常肝、肝硬化、肝血管瘤和肝癌中 IGF- 阳性率分别为 10 % ,76 .9% ,4 6 .4 % ,76 .2 % ;IGFR阳性率分别为2 0 % ,92 .3% ,6 4 .2 % ,6 1.9% .与正常肝组织相比 ,各阳性率有显著性差异 (P<0 .0 5 ) .对于肝癌而言 ,IGF- 及 IGFR的表达在 HBV阳性与阴性之间有显著差异 (P<0 .0 5 ) ;在Edmondson分级 — 与 — 之间有显著差异 (P <0 .0 5 ) .结论 IGF- 和 IGFR的表达可能参与了肝硬化及肝脏肿瘤的发生 .在肝癌中 ,其表达与 HBV感染及肝癌 Ed-m ondson分级亦有一定关系 .

AIM To study the expression of insulin like growth factor Ⅱ (IGF Ⅱ) and its receptor (IGFR) in differ ent types of liver tissues, and to explore the relationship between them and liver cancer's Edmondson grade and infection of HBV. METHODS The expression levels of IGF Ⅱ and IGFR in 10 normal liver specimens, 28 liver angioma specimens, 13 liver cirrhosis specimens and 42 liver cancer specimens were assessed by immunohistochemical techniques. Their positive rates were compared and the relationships between them and HBV infection, Edmondson grade were studied. RESULTS Positive rates of IGF Ⅱ in normal liver,liver cirrhosis,liver angioma and liver cancer were 10%, 76.9%, 46.4% and 76.2% respectively; the positive rates of IGFR, they were 20%, 92.3%, 64.2% and 61.9% respectively. Compared with normal liver tissue, there were significant differences in the positive rates between the normal and abnormal liver tissues ( P <0.05). In liver cancer tissues, the expressions of IGF Ⅱ were significantly different in HBV positive and negative rates ( P <0.05). So were those of IGFR. The expression of IGF Ⅱ in Edmondson grade Ⅰ, Ⅱ and Ⅲ, Ⅳ were significantly differrent ( P <0.05). CONCLUSION Expressions of IGF Ⅱ and IGFR are probably involved in the genesis of liver cirrhosis and liver tumors. Their expressions in liver cancer, to a certain extent, are related to HBV infection and Edmondson grade.