江南卷柏提取物脱脂工艺优选

Optimization of Degreasing Technology of Extracts from Selaginella moelledorfii

目的：优选江南卷柏提取物的石油醚脱脂工艺,为该植物的资源开发与利用提供参考。方法：以穗花杉双黄酮提取量和浸膏得率为评价指标,通过正交试验考察石油醚用量、提取时间和提取次数对脱脂工艺的影响。采用HPLC测定穗花杉双黄酮含量,流动相乙腈（A）-水（B）梯度洗脱（0~5 min,25%A;5~12 min,25%~35%A;12~17 min,35%~45%A;17~25 min,45%~50%A;25~40 min,50%~55%A;40~45 min,55%~70%A;45~50 min,70%~100%A;50~55 min,100%A;55~60 min,100%~25%A）,检测波长330 nm。结果：最佳脱脂工艺为加6倍量石油醚回流提取2次,每次1 h;穗花杉双黄酮提取量72.77 mg,浸膏得率11.04%。结论：该脱脂工艺稳定可行,适用于江南卷柏提取物的纯化。

Objective： To optimize degreasing technology of extracts from Selaginella moelledorfii with petroleum ether, which provides a reference to development and utilization of the plant resources. Method： Taking extraction amount of amentoflavone and yield of extract as evaluation indexes, effects of the amount of petroleum ether, extraction time and frequency on degreasing technology were investigated by orthogonal design. The content of amentoflavone was determined by HPLC, mobile phase consisted of acetonitrile （A） -water （B） for gradient elution （0-5 min, 25%A; 5-12 min, 25%-35%A; 12-17 min, 35%45%A; 17-25 min, 45%- 50%A; 25-40 min, 50%-55%A; 40-45 min, 55%-70%A; 45-50 min, 70%-100%A; 50-55 min, 100%A; 55-60 min, 100%-25% A） and detection wavelength at 330 nm. Result： Optimum technology conditions were as follows： degreased twice with 6 times the amount of petroleum ether for 1 h per time; extraction amount of amentoflavone was 72.77 mg, yield of extract was 11.04%. Conclusion： This degreasing technology is rational and stable, which is available for purification of extracts from S. moelledorfii.