HPLC同时测定灯延颗粒中灯盏乙素、原阿片碱、芹菜素和延胡索乙素

Simultaneous Determination of Scutellarin,Protopine,Apigenin and Tetrahydropalmatine in Dengyan Granules by HPLC

目的：建立HPLC同时测定灯延颗粒中灯盏乙素、原阿片碱、芹菜素和延胡索乙素含量的方法。方法：采用Zorbax Extend-C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈（A）-0.3%醋酸铵水溶液（B）,梯度洗脱（0~25 min,15%A;25~70 min,15%~65%A;70~75 min,65%~15%A）,流速0.8 m L·min^-1,柱温30℃,检测波长分别为280 nm（灯盏乙素、原阿片碱、延胡索乙素）、337 nm（芹菜素）。结果：灯盏乙素、原阿片碱、芹菜素和延胡索乙素进样量分别在0.90~8.99,0.73~7.35,0.05~0.54,0.14~1.37μg线性关系良好;平均回收率分别为99.48%,98.17%,97.41%,98.33%,RSD分别为0.8%,1.2%,1.2%,0.5%。结论：该法适用于同时测定灯延颗粒中灯盏乙素、原阿片碱、芹菜素和延胡索乙素的含量。

Objective： The aim of this study was to develop an HPLC method for the simultaneous determination of contents of scutellarin, protopine, apigenin and tetrahydropalmatine in Dengyan Granules. Method： The analysis was performed on a Zorbax Extend-C18 column （4. 6 mm× 250 ram, 5 μm） in gradient elution （0-25 rain, 15%A; 25-70 rain, 15%-65% A; 70-75 rain, 65%-25% A） by using the mobile phase of acetonitrile （A） -0.3% ammonium acetate solution （B）. The flow rate was 0. 8 mL ·min^-1 and the column temperature was maintained at 30 ℃ , the detection wavelengths were set at 280 nm （for scutellarin, protopine and tetrahydropalmatine） , 337 nm （for apigenin） , respectively. Result ： The calibration curves were linear within the range of 0.90-8.99 Ixg for scutellarin, 0.73-7.35 ixg for protopine, 0.05-0.54 p,g for apigenin and 0. 14-1.37 p,g for tetrahydropalmatine, respectively. The recoveries of scutellarin, protopine, apigenin and tetrahydropalmatine were 99.48% （ RSD 0. 8% ）, 98. 17% （ RSD 1.2% ）, 97.41% （ RSD 1.2% ） and 98.33% （ RSD 0.5% ）, respectively. Conclusion： The method can be used for simultaneous determination of contents of scutellarin, protopine, apigenin and tetrahydropalmatine in Dengyan Granules.