HPLC同时测定山腊梅清感茶中4种黄酮苷的含量

Simultaneous Determination Content of Four Flavonoid Glycosides in Shanlamei Qinggan Cha by HPLC

目的：建立高效液相色谱方法同时测定山腊梅清感茶中4种黄酮苷含量的方法。方法：采用依利特hypersil ODS2色谱柱（4.6 mm×250 mm,5μm）,以乙腈-0.1%冰醋酸为流动相进行梯度洗脱,流速1.0 m L·min^-1,检测波长344 nm,柱温35℃。结果：芦丁在7.012~70.12 mg·L^-1线性关系良好（r=0.999 6）,平均回收率为100.68%,RSD 3.3%,山柰酚-3-O-β-D-半乳糖-（6-1）-α-L-鼠李糖苷在5.126~51.26 mg·L^-1线性关系良好（r=0.999 9）,平均回收率为98.48%,RSD 1.7%,山柰酚-3-O-芸香糖苷在2.631~26.31 mg·L^-1线性关系良好（r=0.999 8）,平均回收率为101.82%,RSD 1.9%,紫云英苷在2.634~26.34 mg·L^-1线性关系良好（r=0.999 9）,平均回收率为97.02%,RSD 1.7%。结论：该方法操作简便,准确,重复性好,可用于山腊梅清感茶的质量控制。

Objective： The aim of this study was to establish an HPLC method for simultaneous determination of the content of four flavonoid glycosides in Shanlamei Qinggan Cha. Method： Using an Elite hypersil ODS2 column （4.6 mm× 250 mm, 5 μm） , the mobile phase was aeetonitrile-0.1% acetic acid, with flow rate of 1.0 mL·min^-1 The detective wavelength was at 344 nm and the column temperature was 35 ℃. Result： The standard curve presented a linear range from 7. 012-70. 12 mg·L^-1 for rutin （r = 0. 999 6） , the average recovery was 100.68% with RSD of 3.27%. The calibration curve was linear in the range of 5. 126-51.26 rag-L-l for kaempferol-3-O-β-D-galactose-（6-1 ）-a-L-rhamnoside （r = 0.999 9）, the average recovery was 98.48% with RSD of 1.7%. The calibration curve was linear in the range of 2. 631-26.31 mg ·L^-1 for kaempferol- 3-O-rutinoside （r = 0. 999 8）, the average recovery was 101.82% with RSD of 1.9%. The standard curve presented a linear range from 2. 634-26.34 mg· L^-1 for astragalin （ r = 0. 999 9） , the average recovery was 97.02% with RSD of 1.7%. Conclusion： The method was fast, accurate and simple in good reproducibility, which could be used to control the quality of Shanlamei Qinggan Cha.