提取溶剂对柴黄颗粒中主要成分的影响

Comparison on Effective Components of Chaihuang Granules Among Three Diffrernt Extracting Solvents

目的:比较不同提取溶剂对柴黄颗粒HPLC图谱及对主要成分色谱峰面积的影响,为改进和完善柴黄颗粒的质量标准提供依据。方法:以HPLC对照品比对法对主要色谱峰进行归属;以检出色谱峰的相对峰面积、色谱峰面积总量,以及主要成分的相对比值及总量对水、甲醇以及70%甲醇等3种提取溶剂的HPLC图谱进行比较分析。结果:共指认了黄芩苷、汉黄芩苷、黄芩素、汉黄芩素等4个色谱峰,3种提取溶剂的HPLC图谱基本一致,但主要色谱峰的峰面积及总峰面积有较大差异,黄芩苷等4种主要成分的相对比值及总量也有较大差异。在建立的色谱条件下,3种提取溶剂的成分含量有明显差异,以水为提取溶剂主要成分含量最高。结论:本研究为改进柴黄颗粒的含量测定方法提供了有益的参考。

Objective：To compare the differences of HPLC profile and the main components among 3 different solvents so as to provide the basis for improving the quality standard of Chai-huang granules.Method：HPLC was employed to identify the main chromatographic peaks,which also was taken to compare the relative peak amounts,total amounts as well as the relative ratio among water,methanol and 70% methanol solvent extracts.Result：Four peaks （baicalin,wogonoside,baicalein,wogonin） were identified.HPLC profiles of 3 extract solvents were almost same,however there were exhibited the significant varieties of the peak amounts,total amounts and the relative ratio of 4 major components.The contents of 3 extract solvents had substantial differences,and water as extracting solvent was highest.Conclusion：This study could offer the useful reference for determination method of Chai-huang granules.