摘要
目的:建立苏黄止咳胶囊中紫苏醛含量的HPLC测定方法。方法:采用高效液相色谱法,SHIMADZU Shim-pack VP-ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水(68:32),检测波长230 nm,流速0.8 m L·min^-1,柱温30℃。结果:紫苏醛在5.672~453.76 ng与其峰面积呈良好的线性关系,线性回归方程为Y=5.695X+1.28(r=0.999 5),平均加样回收率为99.57%(RSD 1.7%)。结论:该方法准确、分离度好、专属性强,可用于苏黄止咳胶囊中紫苏醛的含量测定。
Objective:To establish a method for the content determination of perillaldehyde in Suhuang Zhike Capsule.Method:HPLC was performed on SHIMADZU Shim-pack VP-ODS C18 column (4.6 mm × 250 mm,5 μm),and the mobile phase was consisted of methanol-water (68∶ 32).The detection wavelength was set at 230 nm,the flow rate of 1.0 mL · min^-1 and the column temperature was 30 ℃.Result:Perillaldehyde showed a good linear relationship from 5.672 ng to 453.76 ng,and the regression equation was Y =5.695X + 1.28 (r =0.999 5).The average recovery was 99.57 % with RSD of 1.68 %.Conclusion:The HPLC method is simply and accurate with a good specificity.It can be used to determine the content of perillaldehyde in the Suhuang Zhike Capsule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第24期81-83,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(2010ZX09401-303-2-6)