HPLC测定苏黄止咳胶囊中紫苏醛含量

Determination of Perillaldehyde in Suhuang Zhike Capsule by High-performance Liquid Chromatography

目的：建立苏黄止咳胶囊中紫苏醛含量的HPLC测定方法。方法：采用高效液相色谱法,SHIMADZU Shim-pack VP-ODS C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇-水（68：32）,检测波长230 nm,流速0.8 m L·min^-1,柱温30℃。结果：紫苏醛在5.672~453.76 ng与其峰面积呈良好的线性关系,线性回归方程为Y=5.695X＋1.28（r=0.999 5）,平均加样回收率为99.57%（RSD 1.7%）。结论：该方法准确、分离度好、专属性强,可用于苏黄止咳胶囊中紫苏醛的含量测定。

Objective：To establish a method for the content determination of perillaldehyde in Suhuang Zhike Capsule.Method：HPLC was performed on SHIMADZU Shim-pack VP-ODS C18 column （4.6 mm × 250 mm,5 μm）,and the mobile phase was consisted of methanol-water （68∶ 32）.The detection wavelength was set at 230 nm,the flow rate of 1.0 mL · min^-1 and the column temperature was 30 ℃.Result：Perillaldehyde showed a good linear relationship from 5.672 ng to 453.76 ng,and the regression equation was Y =5.695X ＋ 1.28 （r =0.999 5）.The average recovery was 99.57 % with RSD of 1.68 %.Conclusion：The HPLC method is simply and accurate with a good specificity.It can be used to determine the content of perillaldehyde in the Suhuang Zhike Capsule.