金莲花的薄层鉴别及其总黄酮含量测定

TLC Identification of Trollius chinensis and Its Determination of Total Flavonoids

目的：建立金莲花的薄层鉴别及其总黄酮含量的测定方法,为完善该药材的质量控制体系提供参考。方法：通过单因素试验筛选提取溶剂和展开系统,建立金莲花的TLC。选择荭草苷为指标成分,采用比色法测定总黄酮含量,以10%硝酸铝溶液为显色剂,检测波长510 nm,考察不同产地金莲花中总黄酮含量的差异。结果：展开剂为三氯甲烷-甲醇-丁酮-冰乙酸（4∶2∶3∶3）,建立的TLC分离效果良好。荭草苷线性范围8.096~56.672 mg·L^-1,平均加样回收率为98.8%;1,2,3,4,5,6号样中总黄酮质量分数分别为9.79%,8.71%,8.32%,6.47%,6.42%,6.71%。结论：建立的金莲花定性鉴别和定量检测方法简便、准确,适用于该药材的质量控制。

Objective：To establish a TLC Identification of Trollius chinensis and determination of total flavonoids in the herb.Method：Extraction solvent and deployment system in TLC identification method of T.chinensis was screened by single factor tests.A colorimetry was adopted to determine the content of total flavanoids in T.chinensis by taking orientoside as index component,10% aluminum nitrate solution as color developing reagent and detection wavelength at 510 nm,then content difference of total flavanoids in T.chinensis from different origins was compared.Result：Developing agent was chloroform-methanol-butanone-acetic acid （4∶ 2∶ 3∶ 3）,this established TLC had a good separation effect of each component.Orientoside had a good linear relationship in the range of 8.096-56.672 mg · L^-1,average recovery was 98.8% ; the mass fractions of total flavanoids in No.1,2,3,4,5,6 sample were 9.79%,8.71%,8.32%,6.47%,6.42% and 6.71%,respectively.Conclusion：These methods are simple,accurate and applicable for quality control of T.chinensis.