黑果枸杞含片体内抗氧化作用

Study on Antioxidant Effect of Lycium ruthenicum Buccal Tablet in vivo

目的:研究黑果枸杞含片的体内抗氧化作用。方法:40只小鼠随机分为4组:正常组、黑果枸杞含片低、高剂量组(0.08,0.16 mg·g-1)和阳性药组(维生素C,0.02 mg·g-1)。各组分别连续ig给药28 d后眼眶采血及取肝组织,试剂盒测定小鼠血清和肝脏中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、总抗氧化能力(T-AOC)和过氧化氢酶(CAT)活性。结果:与正常组比较,黑果枸杞含片低、高剂量组小鼠的体重没有明显变化,小鼠血清和肝组织中MDA分别为(5.97±2.53),(11.08±3.72)μmol·L-1和(5.84±1.44),(10.79±2.06)μmol·L-1与正常组(8.69±2.85)μmol·L-1,(14.49±1.56)μmol·L-1相比显著降低(P<0.05,P<0.01),T-AOC,SOD,GSH-Px和CAT活性明显提高(P<0.05,P<0.01),尤其是高剂量组T-AOC,SOD,GSH-Px和CAT活性显著提高。结论:黑果枸杞含片具有显著的体内抗氧化活性。

Objective：To establish mice model of functional dyspepsia （FD） and to investigate the effects of Weiyuanning on gastrointestinal motility and gastrointestinal hormones.Method：The mice were randomly divided into normal group,model group,low-,medium-and high-dose Weiyuanning groups （22.5,45,90 g ·kg^-1）,and domperidone group （10 mg ·kg^-1）.FD models were made by irregular food intake plus intraperitoneal injection with L-arginine in mice except for mice in normal group.All mice were ig administrated with corresponding medicines for 7 days.The body weights,the gastric remnant rate and small intestinal propulsion rate were measured.The gastric histopathological changes were observed.The contents of gastrin （GAS） and motilin （MTL） were detected by Elisa.Result：Compared with model group,the body weight,gastric emptying rate were decreased,the small intestinal propulsion rate was increased in model group （P 〈 0.01）.The above indexes were improved in the Weiyuanning groups （P 〈 0.01）.Meanwhile,the the contents of GAS in serum and MTL in plasma were increased in Weiyuanning groups （P 〈 0.05,P 〈 0.01）.No significant changes were found in the improvement of gastric histopathological among all groups after the treatment.Conclusion：FD model could be successfully made by irregular food intake plus intraperitoneal injection with L-arginine.Weiyuanning has significant effect in improving indigestion in FD mice.Its mechanism may be related to elevating the levels of GAS in serum and MTL in plasma of mice.