骨髓间充质干细胞移植对大鼠重症急性胰腺炎的治疗作用观察

Treatment effect of bone marrow mesenchymal stem cells transplantation on the severe acute pancreatitis in rats

目的探讨骨髓间充质干细胞(MSCs)移植对重症胰腺炎(SAP)的治疗作用及相关机制。方法将雄性SD大鼠120只随机分为假手术组、模型组及移植组各40只。模型组和移植组采用5%牛磺胆酸钠逆行胰胆管注射方法制作SAP模型,假手术组开腹后翻动肠壁即关腹。造模成功后移植组经门静脉植入MSCs;模型组及假手术组采用同样方法给予等量生理盐水。于24、48、72 h每组分别处死10只大鼠,对胰腺和肾脏损伤进行大体形态和光镜下观察,测定血清淀粉酶(AMY)、BUN、Cr、TNF-α和IL-6水平。结果模型组胰腺和肾脏组织损伤明显,各时间点血清AMY、BUN、Cr、TNF-α和IL-6水平均高于假手术组,P均<0.05。移植组胰腺和肾脏组织损伤有所减轻;各时间点血清中AMY、BUN、Cr、TNF-α和IL-6水平均低于模型组及假手术组,P均<0.05。结论经门静脉移植MSCs治疗SAP效果确切,可降低多器官功能障碍的发生率。

Objective To study the protective effect and the possible mechanisms of transplantation bone marrow mesenchymal stem cells（ MSCs） on pancreatic injury in rats with severe acute pancreatitis（ SAP）. Methods A total of 120 male Sprague-Dawley rats were randomly divided into sham operation group（ N group）,severe acute pancreatitis group（ P group）,transplantation MSCs group（ M group）. The dose of 5% sodium taurocholate was retrograde injected into the pancreatic duct of the rats in P group and M group to induce SAP model. Rats in N group whose pancreases were just flipped and striked gently without injection. MSCs were transplanted into rats in M group via portal vein,while the same dosage of0. 9% NS was injected by the same vein routine in P group. Ten rats in each group were sacrificed at 24 h,48 h,72 h later. Pancreas and kidney damage of each group were observed under gross morphology and optical lens. The serum AMY,BUN,Cr,TNF- α and IL-6 level of each group were tested. Results Pancreas and kidney injury in P group were significantly heavier. The content of serum AMY,BUN,Cr,TNF- α and IL-6 level in P group were both higher than those of N group（P〈0. 05）. Pancreatic and renal tissue injury in M group rats mitigated. The content of serum AMY,BUN,Cr,TNF- α and IL-6 level in M group were both lower than those of P group（P〈0. 05）. Conclusions Transplantation via portal vein MSCs is an effective treatment on SAP,which can reduce the occurrence of multiple organ dysfunction and improve prognosis.