UMOD基因启动子区单核苷酸多态性与CKD患者发病年龄的关系

Relationship between single nucleotide polymorphisms in UMOD gene promoter region and the age-onset of chronic disease kidney

目的：探讨UMOD基因启动子区多态性与慢性肾脏病患者发病年龄的关系。方法选取89例CKD患者作为病例组，选择与病例组年龄、性别相匹配的体检健康者104例作为对照组。采用PCR法检测两组UMOD基因启动子区进行扩增并测序，分析其多态性与CKD患者发病年龄的关系；分析性别、吸烟史、体重指数（ BMI）、平均动脉压等与CKD患者发病年龄的关系。结果病例组－1617（ rs13333226） G等位基因的频率高于对照组（χ^2＝4．737，P＝0．030）；携带rs13333226A者发病年龄早于携带rs13333228G患者（χ^2＝7．902，P＝0．005）。单因素分析结果显示，BMI≥22的CKD患者发病年龄＜45岁者的比例高于BMI ＜22者，吸烟者发病年龄＜45岁者的比例高于非吸烟者（P均＜0．05）。多因素分析示UMOD基因rs13333226的多态性是CKD患者发病年龄的独立影响因素（OR＝0．494，95％CI：0．268-0．913，P＜0．05）。结论 CKD患者UMOD基因rs13333226等位基因的频率增高，携带rs13333226 A的CKD患者发病年龄较小。 UMOD基因rs13333226的多态性是CKD患者发病年龄的独立影响因素。

Objective To investigate the relationship between single nucleotide polymorphisms in UMOD gene promoter region and the age-onset of chronic kidney disease （ CKD ） .Methods A total of 89 CKD patients （ patient group ） and 104 healthy volunteers （ control group ） were involved in this study .PCR was used to detect the peripheral blood promoter region of UMOD gene .The relationship between the polymorphisms in UMOD gene promoter and the age-onset of chronic disease kidney was analyzed .The relationship between the age-onset of CKD and sex, age, smoking history, BMI, mean arterial pressure were analyzed .Results The sequence of -1 617（rs13333226）G polymorphisms in patient group was higher than that in control group （χ^2 =4.737, P=0.030）.The onset age of CKD patients with rs13333226A was significantly younger than that of patients with rs13333226G（χ^2 =7.902, P=0.005）.Single factor analysis showed that the age-onset of CKD patients with BMI〉22 was earlier than those with BMI〈22（〈0.05）.The age-onset of CKD patients with smoking history was earlier than those without smoking history （〈0.05）.The SNP of rs13333226 was the independent risk factor for the the age-onset of CKD（OR=0.494,95%CI：0.268~0.913,〈0.05）.Conclusions The frequency of rs13333226 G allele was higher in CKD patients .The onset age of CKD patients with rs 13333226 A was significantly earlier.The SNP of rs13333226 was the independent risk factor for the the age-onset of CKD.