东乡野生稻的粒形相关QTL分析

QTL Analysis for Grain Shape Traits of Dongxiang Wild Rice

以东乡野生稻/协青早B∥协青早B的回交自交系群体为遗传材料,利用简化基因组测序技术鉴定群体的SNP分子标记基因型,对东乡野生稻的粒形相关性状进行QTL分析。在水稻第1,3,4,5,10,12号染色体上共发现14个粒形相关QTL,包括6个粒长QTL(q GL1、q GL3.1、q GL3.2、q GL4、q GL10.1、q GL10.2)、2个粒厚QTL(q GT5、q GT10)和6个粒重QTL(q KGW3、q KGM4、q KGW5、q KGW 10.1、q KGW10.2、q KGW12)。其中q GL3.1与q KGW3、q GT5与q KGW5、q GL4与q KGM4、q GL10.1与q KGW10.1以及q GL10.2、q GT10与q KGW10.2定位区间重叠,推测这些QTL具有一因多效特性。加性效应最大的粒长QTL即q GL3.1(q KGW3)被定位在第3染色体的粒形基因GS3的区域中,且q GL3.1与GS3都是控制粒长的主效QTL,推测q GL3.1就是GS3基因。而在q GL3.1区域附近还存在粒长QTL q GL3.2,该位点与已克隆的粒长QTL即GL3.1在位置上重叠,推测q GL3.2可能就是GL3.1/Os PPKL1基因。

Using the SLAF-seq（specific-locus amplified fragment sequencing） for genotyping, the QTL analysis for grain shape traits was conducted in the population of backcross inbred lines（BIL） of Dongxiang wild rice（ O. ruff- pogon, DXWR）/Xieqingzao B （XQZB）//XQZB. The results showed that 14 QTLs of grain shape traits were detec- ted on Chromosomes 1, 3, 4, 5, 10 and 12, among which, there were six grain length QTLs（qGL1, qGL3. 1, qGL3.2, qGL4, qGL10. 1, qGL10. 2 ）, two grain thickness QTLs （ qGT5, qGT10） and six grain weight QTLs （ qKGW3 , qKGW4 , qKGW5 , qKGW10. 1, qKGW10. 2, qKGW12 ）. The marker intervals of QTLs were overlapped between qGL3.1 and qKGW3 , qGT5 and qKGWS , qGL4 and qKGW4 , qGL10. 1 and qKGW10. 1, and among qGL10. 2, qGT10 and qKGW10. 2, indicating that these QTLs tended to have pleiotropism, qGL3.1 had the greatest addi- tive effect of grain length in this study and was located in the region of GS3, a cloned grain shape gene, on Chromo- some 3, and both of them were major QTLs for grain length, so it was concluded that GS3 was the candidate of qGL3.1. Besides, qGL3.2, another grain length QTL, was located near qGL3. 1, where lay GI3. 1/OsPPKL1, a cloned grain length QTL, suggesting that GL3. 1 was the candidate of qGL3. 2.