异氰酸酯作业工人诱导痰炎性细胞及血清IL-8和IL-1β水平的变化

Changes of Inflammatory Cells in Induced Sputum and IL-8,IL-1β Levels in Serum of Workers Exposed to Isocyanate

目的研究异氰酸酯作业工人气道炎症情况及相关细胞因子的变化。方法选择某发泡车间55名异氰酸酯作业工人为实验组,进行问卷调查、呼气峰值流速(PEF)监测及诱导痰细胞学分类计数。选择某体检中心33名健康受检者为对照组。应用ELISA测定2组受试者血清白细胞介素8(IL-8)及IL-1β水平。结果实验组受试者班后诱导痰中性粒细胞百分数较班前增高(P<0.05),巨噬细胞百分数较班前降低(P<0.05),嗜酸性粒细胞百分数较班前降低(Z=-2.00,P<0.05),差异有统计学意义,淋巴细胞无显著变化。实验组受试者血清IL-8浓度高于对照组(Z=-4.025,P<0.01),IL-1β浓度低于对照组(Z=-4.279,P<0.01)。实验组受试者血清IL-8水平与诱导痰中性粒细胞变化呈正相关(r=0.29,P<0.05)。结论异氰酸酯作业工人在接触异氰酸酯后气道发生以中性粒细胞为主的炎性细胞浸润,中性粒细胞被激活并参与到致敏过程中。

Objective To investigate the airway inflammation of workers exposed to isoeyanate, and analyze the changes of related cytokines in se-rum. Methods A total of 55 isocyanate-exposed workers were recruited as the experimental group and examined by questionnaire.PEF and sputum induction were measured before and after work. 33 health individuals were selected as control group. Serum IL-8, IL-1β levels of all subjects were de-leered by EILSA. Results Neutrophil percentage in the induced sputum was increased （ P 〈 0.05 ）, maerophage percentage in the induced sputum was decreased （ P 〈 0.05 ）, and eosinophil percentage in the induced sputum was decreased（ Z = -2.00, P 〈 0.05 ）after work. There was no statistical significance in the changes of lymph cells. The serum IL- 8 levels of experimental subjects were higher than those of control gruup （Z =-4.025, P 〈 0.01 ）, 1L- 1β levels of experimental subjects were lower than those of control group （ Z =-4.279, P 〈 0.01 ）. Serum IL-8 level of experimental sub- jects was positively correlated with the difference of neutrophil cells in induced sputum （ r =0. 29, P 〈 0.05 ）. Conclusion Neutrophil cells were ac-tivated and participated in the inflammatory infiltration of airway after exposure to isocyanate.