植物病原细菌DNA条形码检测技术

DNA barcoding technology for plant associated bacteria detection

植物病原细菌是植物病原菌的重要组成部分,DNA条形码技术在植物病原细菌的鉴定中应用非常普遍,本文综述了在植物病原细菌鉴定中常用的基因及目前应用的一些数据库,认为在细菌鉴定中比较适用的方法为先通过16S r RNA基因分析确定到属,然后通过多态性更强的基因或多个基因的联合应用鉴定到种或种下阶元。DNA条形码技术可以作为常规检测技术的一个强有力补充,提高病原菌鉴定的标准化与速度,尤其对检疫性病原细菌的鉴定具有重要作用。

DNA barcoding technology plays an important role in identification of phytopathogens. This paper reviews the commonly used DNA barcoding genes and online databases for detecting and identifying plant associated bacteria. Here we recommend a method and consider it a more suitable way for bacteria identification, that is, to first determine its genus by 16S rRNA, then determine its species as well as its subtypes using one or more genes that are more polymorphic. DNA barcoding technology can be used to improve the efficiency of pathogen detection and to speed up the standardization process, especially when for detecting quarantine pathogens. Thus it’s a powerful complement for conventional detection techniques.