马铃薯试管苗快繁培养基的优化研究

Medium Optimization for Potato Plantlet Culture

针对马铃薯试管苗快繁继代繁殖周期长和移栽后成活率低的实际问题,以马铃薯品种克新1号脱毒试管苗为试验材料,采用多因子正交试验L9(34)设计方法,以MS培养基中4种无机盐Ca Cl2、Ca(NO3)2、KH2PO4、KNO3为试验因素,研究不同无机盐浓度对马铃薯试管苗生长的影响。结果表明,在Ca Cl2浓度为0mmol/L、Ca(NO3)2浓度为20mmol/L、KH2PO4浓度为6mmol/L、KNO3浓度为18mmol/L条件下,克新1号马铃薯试管苗的株高、茎粗、根系、茎叶鲜重生长达到最好。本研究结果可为马铃薯组培快繁过程中培养基制备提供依据。

The effect of mineral salt coneentrationson potato plantlet growth were studied to solve the problems about the long reproductive cycle and low survival rate for the potato plantlet culture. Adopted multi-factor orthogo- nal experiment L9 （3^4） design was used to change the MS medium concentration of CaCl2, Ca（ NO3 ）2, KH2PO4, KNO3 to screen the optimum concentration for potato tissue culture medium preparation. The results showed that CaCl2 0mmol/L, Ca（ NO3 ）2 20mmol/L, KH2PO4 6mmol/L, KNO3 18mmol/L was the best combination for the po- tato planflet culture medium.