枇杷内生木霉P3.9菌株抗菌谱研究

Study on Antimicrobial Spectrum of Trichoderma P3.9 Strain of Endophytic Fungus from Loquat

以枇杷内生木霉P3.9菌株及枇杷根腐病病菌Pestalotiopsis sp.、康乃馨根腐病菌Fusariumsp.、仙客来根腐病菌Fusariumsp.、万寿菊叶斑病病菌Alternariasp.、辣椒黑斑病病菌Alternariasp.、辣椒黄萎病病菌Verticillium dahliae 6种供试病原真菌为试材,采用对峙培养法,将P3.9菌株与各病原菌进行对峙培养,测定其对各病原真菌的抑制作用。结果表明:P3.9菌株对6种供试病原真菌均有不同程度的抑制作用,抑制率分别为80.6%、67.6%、73.1%、77.5%、82.0%和82.7%,抑制率均在60%以上,说明P3.9菌株抗菌谱广泛,表现出良好的开发应用前景,为有效可持续防控上述各病害提供参考依据及生防菌种资源。

Taking Trichoderma P3.9 strain of endophytic fungus from loquat,the pathogen of loquat root rot（Pestalotiopsis sp.）,carnation root rot（Fusariumsp.）,florist＇s cyclamen root rot（Fusariumsp.）,marigold leave spot（Alternariasp.）,pepper black spot（Alternaria alternata）and pepper yellow blight（Verticillium dahliae）as materials,the pathogens were respectively inoculated with P3.9strain by dual culture,inhibition effect of P3.9strain on different pathogens were determined.The results showed that P3.9strain had different antifungal activity to the above pathogens.The inhibition rate was respective 80.6%,67.6%,73.1%,77.5%,82.0% and 82.7%.All were more than 60% and antimicrobial spectrum of P3.9strain was wide.It possessed a good prospect in the development and application.It provided theoretical basis and strain resources for bio-control to the above diseases.