粉防已碱对血管平滑肌细胞增殖及相关基因表达的影响

Effect of tet on proliferation and relevant gene expression of vascular smooth muscle cells

目的 :研究粉防已碱 (Tetrandrine,Tet)对内皮素诱导的大鼠血管平滑肌细胞 (VSMC)增殖及相关基因表达的影响。方法 :采用细胞计数及3H TDR掺入实验检测VSMC增殖状态 ;应用Northern杂交检测Tet对VSMC诱导型一氧化氮合酶 (iNOS)基因和ET诱导的c jun基因表达的影响。结果 :Tet可明显抑制VSMC的增殖 (P <0 0 5 ) ,抑制ET对VSMC的促丝裂作用 (P <0 0 5 ) ;抑制原癌基因c jun的表达 ,促进iNOS转录。结论 :Tet可能通过调节iNOS和细胞增殖相关基因的表达而抑制VSMC的增殖。

Objective:To study the effect of Tet on the proliferation and relevant gene expression of vascular smooth muscle cells(VSMC).Method:Using cell counting and 3H\|TDR incorporation to investigate the VSMC proliferation,using Northern blotting to detect the effect of Tet on induced NO synthase(iNOS),endothelin(ET)induced c\|jun gene expression.Result:The cell counting and 3H\|TDR incorporation value of VSMC treated by Tet was lower than that of control group( P <0\^05).rich iNOS mRNA was detected in the VSMC stimulated by Tet.Expressions of c\|jun by ET in the VSMC were significantly inhibited by Tet.Conclusion:The Tet could significantly inhibit VSMC proliferation. [