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PRG4蛋白SMB结构域的表达及聚集现象研究

Research on Expression of Somatomedin B Domain of Proteoglycan 4 and Recombinant Protein Aggregation
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摘要 利用糖蛋白4(PRG4)分子多态性的特点,克隆表达含有两个人促细胞生成素B(SMB)结构域及少量糖基化重复序列的重组蛋白(SMBPRG4),并建立了成熟的纯化工艺流程,可高效表达重组蛋白。进一步通过凝胶排阻、动态光散射实验发现重组蛋白具有自我聚集的特点,在溶液中以稳定的二聚体形式存在。结构预测及非还原电泳揭示SMBPRG4是非共价结合的同源二聚体。 Recombinant protein SMBPRG4 containing two Somatomedin B domains and a small amount of glycosylation of repetitive sequences of proteoglycan 4 was cloned according to PGR4 gene polymorphism. Mature purification process was established and recombinant protein SMBPRG4 , with high-level expression was purified. By using size ex- clusion chromatogaraphy and dynamic light scattering, we found that the recombinant protein self-aggregate to di- meric form. Structure prediction and non reducing electrophoresis revealed that SMBPRG4 was a non-covalently bonded dimer.
作者 王黎芳 韩之波 陈文虎 杜蓬 孙爱华 杨萍 赵宏光
机构地区 浙江医学高等专科学校基础医学部生化教研室 中国协和医科大学血液学研究所 浙江肿瘤医院胸外科
出处 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2014年第6期1319-1324,共6页 Journal of Biomedical Engineering
基金 浙江省卫生高层次创新人才培养工程资助项目 浙江省自然科学基金资助项目(LQ12H16001)
关键词 糖蛋白4 基因表达 结构 蛋白聚集 proteoglyean 4 gene expression structure protein aggregation
分类号 Q51 [生物学—生物化学]
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参考文献15

  • 1DENG G, ROYLE G, WANG SJ, et al. Structural and functional analysis of the plasminogen activator inhibitor-1 binding motif in the somatomedin B domain of vitronectin[J].J Bioi Chern , 1996,271(22): 12716-12723.
  • 2ZHUANG p, BLACKBURN M N, PETERSON C B. Characterization of the denaturation and renaturation of human plasma vitronectin. I. Biophysical characterization of protein unfolding and multimerization[J].J Bioi Chern , 1996, 271 (24): 14323-14332.
  • 3GUSBERS R, CEULEMANS H, BOLLEN M. Functional characterization of the non-catalytic ectodomains of the nucleotide pyrophosphatase/phosphodiesterase NPP1[J]. BiochemJ, 2003, 371(Pt 2): 321-330.
  • 4IKEGAWA S, SA NO M, KOSHIZUKA v , et al. Isolation, characterization and mapping of the mouse and human PRG4 Cproteoglycan 4) genes[J]. Cytogenet Cell Genet, 2000, 90 (3/4): 291-297.
  • 5JAY G D, TANTRAVAHI U, BRITT D E, et al. Homology of lubricin and superficial zone protein (SZP): products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondrocytes localized to chromosome 1q25[J].J Orthop Res, 2001, 19 (4): 677-687.
  • 6REES S G, DAVIESJ R, TUDOR D, et al. Immunolocalisation and expression of proteoglycan 4 (cartilage superficial zone proteoglycan) in tendon[J]. Matrix Bioi, 2002, 21(7): 593-602.
  • 7PANAGOPOULOS t, MERTENS F, ISAKSSON M, et al. Expression of DOL54 is not restricted to myxoid liposarcomas with the FU5-DDIT3 chimera but is found in various sarcomas[J]. Oncol Rep, 2004, 12(1): 107-110.
  • 8SNAPP K R, CRAIG R, HERRON M, et al. Dimerization of P-selectin glycoprotein ligand-I (PSGL-1) required for optimal recognition of P-selectin[J].J Cell Bioi, 1998, 1420): 263-270.
  • 9EPPERSON T K, PATEL K D, MCEVER R r. et al. Noncovalent association of P-selectin glycoprotein ligand-I and minimal determinants for binding to P-selectin[J].J Bioi Chern, 2000, 275(11): 7839-7853.
  • 10AFSHAR-KHARGHAN V, DIZ-KUCUKKAYA R, LUDWIG E H, et al. Human polymorphism of P-selectin glycoprotein ligand 1 attributable to variable numbers of tandem decameric repeats in the mucinlike region[J]. Blood, 2001, 97(10): 3306-3307.
生物医学工程学杂志
2014年 第6期

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