生长因子对人脂肪干细胞成软骨诱导作用研究

Growth Factors-mediated Effects on the Differentiation of Human Adipose-derived Stem Cells into Chondrocytes

人脂肪干细胞(hADSCs)的成软骨潜能是近年来组织工程学研究的热点之一,而生长因子是诱导hADSCs向软骨细胞分化的关键因素。目前用于软骨诱导的生长因子多来自转化生长因子(TGF)-β超家族,其中以TGF-β1/3最为常见。但hADSCs与人骨髓干细胞(hBMSCs)对TGF-β的反应存在差异,且研究发现,骨形态发生蛋白-6(BMP-6)可以显著上调hADSCs的TGF-βⅠ型受体的表达水平,因此传统的hBMSCs软骨诱导液未必同样适合hADSCs。于是本文结合近年来的相关研究,对用于hADSCs成软骨诱导的生长因子做一综述。

In recent years, there has been a growing emphasis on use of human adipose-derived stem cells （hADSCs） for cartilage tissue engineering owing to their ability to differentiate into chondrocytes, which is mainly induced by growth factors （GFs）. In general, GFs for chondrogenic induction come from the transforming growth factor β （TGF-β） superfamily. To date, the most commonly used GFs for chondrogenes is TGF-β1/3. However, the response of hADSCs to GFs may differ significantly from that of human bone marrow stem cells （hBMSCs）. It has been reported that bone morphogenetic protein-6 （BMP-6） treatment induced TGF-β receptor-Ⅰ expression of hADSCs. It seems that these two ceil populations varied strongly in their potency to undergo chondrogenesis in the same medium conditions. Here, we provide a concise review on various GFs used in chondrogenic differentiation of hADSCs in vitro.