创伤性脑损伤大鼠脂肪组织抵抗素水平与糖代谢的相关性研究

Correlation between expression of resistin in adipose tissues and glycometabolism in rats with traumatic brain injury

目的 探讨大鼠创伤性脑损伤（traumatic brain injury,TBI）后抵抗素在皮下脂肪组织中的表达规律及其对糖代谢的影响.方法 56只SD大鼠按随机数字表法分为TBI组和对照组.TBI组包括TBI后12,24,72h、1,2,4周6个时相点,每时相点8只;对照组8只.采用实时PCR测定大鼠皮下脂肪组织抵抗素mRNA的表达,Westem blot印迹法测定皮下脂肪组织抵抗素蛋白表达,ELISA法测定血清胰岛素、空腹血糖并计算定量胰岛素敏感性检测指数,对上述指标及其之间的相关性进行统计学分析.结果 TBI组大鼠皮下脂肪抵抗素表达、血清胰岛素和空腹血糖水平均明显高于对照组（P＜0.05）,定量胰岛素敏感性检测指数低于对照组（P＜0.05）.单因素直线相关分析显示,TBI抵抗素基因表达与定量胰岛素敏感性检测指数呈显著负相关（P＜0.05）.结论 TBI大鼠皮下脂肪组织抵抗素表达明显增高,且与糖代谢呈负相关,抵抗素可能在TBI后发生胰岛素抵抗过程中发挥重要作用.

Objective To investigate the expression pattern of resistin in subcutaneous adipose tissue and its effect on glycometabolism in rats with traumatic brain injury （TBI）.Methods Fifty-six SD rats were randomly divided into TBI group （n =48） and control group （n =8） according to the random number table.mRNA and protein expressions of resistin in subcutaneous adipose tissue were detected with real-time PCR and Western-blot.Concentrations of serum insulin and serum fasting blood glucose were evaluated using the ELISA method and quantitative estimation of insulin sensitivity was performed.Indices measured and their correlations were statistically analyzed.Results Levels of the resistin,serum insulin and FBG were significantly higher in TBI group than in control group （P 〈 0.05）.Quantitative estimation of insulin sensitivity lowered in TBI group compared to control group （P 〈 0.05）.Single factor linear correlation analysis showed negative correlation between resistin expression and quantitative insulin sensitivity check index in TBI group （P 〈 0.05）.Conclusions Resistin is shown to have significant expression in subcutaneous adipose tissue and involve in glycometabolism.Obviously,resistin may play a significant role in insulin resistance after TBI in rats.