出血性卒中病人急性期外周血CD34^+和CD133^+/CD34^+细胞动员及其意义

Mobilization of CD34^+ and CD133^+/CD34^+ cells in peripheral blood of patients with acute hemorrhagic stroke and its significance

目的探讨出血性脑卒中病人急性期外周血CD34+和CD133+/CD34+细胞动员情况及其意义。方法选取20例出血性脑卒中病人(出血组)和15名健康体检者(对照组),检测两组外周血中CD34+和CD133+/CD34+细胞数。结果出血组的CD34+细胞与CD133+/CD34+细胞在卒中后1~7 d均低于对照组(均P<0.05)。与1 d时相比,出血组外周血CD34+细胞数在卒中后2 d急剧下降,3 d时开始逐渐上升,5 d时达到最大,随后逐渐下降;CD133+/CD34+细胞数从卒中后1 d时开始逐渐升高,5 d时达到最大,随后逐渐下降。结论出血性卒中病人急性期外周血CD34+和CD133+/CD34+细胞均出现动员,CD34+和CD133+/CD34+细胞数量变化情况可能是急性出血早期敏感指标。

Objective To investigate the mobilization of CD34＋ and CD 133~/CD34~ progenitor cells in peripheral blood of patients with acute hemorrhagic stroke and its significance. Methods Twenty patients with acute hemorrhagic stroke （H group） and 15 healthy people （control group） were selected. CD34^＋ and CD133＋/CD34^＋ progenitor cells in peripheral blood were counted in the two groups. Results After hemorrhagic stroke 1-7 d, CD34^＋ and CD133＋/CD34^＋ cells in H Group were lower than those in control group （all P 〈 0.05）. In the H group, compared with ld, the CD34^＋ cells there were sharp decline at 2rid d, CD34＋ cells were gradually increased from 3rd d, reached a maximum at 5th d, and then gradually decreased. CD133^＋/CD34^＋ cells began to rise from 1 d, reached the maximum at 5 d, and then gradually decreased. Conclusions CD34^＋ and CD133＋/CD34^＋ cells in peripheral blood are mobilized at early stage in the patients with hemorrhagic stroke. The changes in number ofCD34^＋ and CD133^＋/CD34^＋ might be a sensitive indicator for early acute hemorrhage. Key words： cerebral hemorrhage; endothelial progenitorcells; CD34^＋; CD133＋/CD34^＋