摘要
目的通过研究中药复方Formula-3(乌梅、灵芝、人参、当归、黄连、干姜)对嗜碱性粒细胞系(RBL-2H3细胞)和SOC通道的作用,探讨Formula-3对肥大细胞脱颗粒的作用机制。方法体外培养大鼠RBL-2H3细胞系制作肥大细胞的模型,通过MTT实验、脱颗粒实验和激光共聚焦(Confocal)实验来研究Formula-3对肥大细胞脱颗粒的作用机制。结果 Formula-3(终质量浓度0.05、0.2、0.8 mg/ml)在急性(0.5 h)和慢性(12 h)作用下呈质量浓度依赖性的抑制致敏RBL-2H3细胞脱颗粒(P<0.01),而对非致敏RBL-2H3细胞没有影响(P>0.05)。Formula-3可显著的抑制SOC通道钙离子内流(P<0.01)。结论 Formula-3通过抑制SOC通道钙离子内流来显著抑制肥大细胞脱颗粒,为治疗过敏性疾病提供理论依据。
In order to explore the mechanism of Herbal Formula-3(consists of Fructus pruni mune,Ganoderma Lucidum, Radix ginseng, Corpus radix angelicae sinensis, Rhizoma coptidis and Rhizoma zingiberis officinalis) in inhibiting mast cells degranulation, we cultured RBL-2H3 cell lines in vitro and investigated the effects of Formula-3 on RBL-2H3 cells and SOC channel by MTT and Confocal Methods. We found that Formula-3(final concentration 0.05, 0.2 and 0.8 mg/ml respectively) could inhibit sensitized RBL-2H3 cells degranulation acutely(0.5 h) and chronically(12 h) in a dose-dependant manner(P 〈 0.01), and Formula-3 could significantly inhibit calcium influx of SOC channel(P 〈 0.01), but there were no effect on non-sensitized RBL-2H3 cells(P 〉 0.05). The results suggested that mast cells degranulation can significantly inhibited by Formula-3 through decreasing calcium mobilization of SOC channel, which provided a theoretical basis for the treatment of allergic diseases.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第12期1044-1047,共4页
Immunological Journal
基金
国家自然科学基金(81373176
81271950
31101280)
广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009)
深圳市科技计划国际科技合作项目(GJHZ20130408174112021)