摘要
A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of lycorine and galanthamine, two major constituents in Lycoris radiata extract, in rat plasma. Liquid-liquid extraction with ethyl ether was carried out using diphenhydramine as the internal standard The two bioactive alkaloids were separated on a Zorbax SB-C18 reserved-phase column （150 mm× 4.6 mm, i.d., 5 μm） by gradient elution using a mobile phase consisting of methanol with 0.1% formic acid （A） and water with 0.1% formic acid （B） at a flow rate of 0.6 mL/min. All analytes showed good linearity over a wide concentration range （r^2〉0.99） and the lower limit of quantification was 3.00 ng/mL for each analyte. The average extraction re- covery of the analytes from rat plasma was more than 82.15%, and the intra-day and inter-day accuracy and preci- sion of the assay were less than 12.6%. The validated method was successfully applied to monitoring the concen- trations and pharmacokinetic studies of two Amaryllidaceous alkaloids in rat plasma after an oral administration of Lycoris radiata extract.
A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of lycorine and galanthamine, two major constituents in Lycoris radiata extract, in rat plasma. Liquid-liquid extraction with ethyl ether was carried out using diphenhydramine as the internal standard The two bioactive alkaloids were separated on a Zorbax SB-C18 reserved-phase column （150 mm× 4.6 mm, i.d., 5 μm） by gradient elution using a mobile phase consisting of methanol with 0.1% formic acid （A） and water with 0.1% formic acid （B） at a flow rate of 0.6 mL/min. All analytes showed good linearity over a wide concentration range （r^2〉0.99） and the lower limit of quantification was 3.00 ng/mL for each analyte. The average extraction re- covery of the analytes from rat plasma was more than 82.15%, and the intra-day and inter-day accuracy and preci- sion of the assay were less than 12.6%. The validated method was successfully applied to monitoring the concen- trations and pharmacokinetic studies of two Amaryllidaceous alkaloids in rat plasma after an oral administration of Lycoris radiata extract.
基金
supported by the Natural Science Foundation of Fujian Province of China(No.2013J01382)
