期刊文献+

β2-GP1、VEGF、TF与大鼠DVT形成的关系 被引量:3

The Correlation between β2-GP1,VEGF and TF with Rat DVT Formation
下载PDF
导出
摘要 目的建立大鼠下腔静脉狭窄法深静脉血栓(DVT)模型,检测大鼠血液中β-2糖蛋白1(β2-GP1)、血管内皮生长因子(VEGF)、组织因子(TF)表达变化,研究其与DVT形成的关系。方法 70只SD大鼠,随机分为对照组(10只),假手术组(30只),模型组(30只),模型组于造模后2 h、8 h、24 h分为3个亚组,采集各组大鼠下腔静脉血5 m L,ELISA法检测β2-GP1、VEGF、TF水平。结果大鼠血液中β2-GP1、VEGF、TF表达在对照组与假手术组间比较无统计学差异(P〉0.05),造模后2~24 h,β2-GP1、VEGF、TF在血液中的表达呈上升趋势,24 h时达峰值,高于对照组、假手术组、造模后2 h组和造模后8 h组(P〈0.01),其余4组间比较差异无统计学意义(P〉0.05)。结论大鼠DVT形成过程中,β2-GP1、VEGF、TF可能起到促进血栓形成的作用。 Objective To build rat DVT inferior vena cava partial stasis(narrow) model, to detected the expressionof β2-GP1, VEGF and TF in rat blood, and to investigat the correlation between β2-GP1, VEGF and TF with DVT. Methods SD rats(n=70) are divided into control group(n = 10), sham operation group(n = 30) and the model group(n = 30) ran-domly and DVT model was built by the inferior vena cava partial stasis(narrow) after 2 h, 8 h and 24 h respectively. In eachtime point, ten rats were taken in each group, inferior vena cava blood were collected while β2-GP1, VEGF and TF expres-sion were detected by ELISA. Results In rat experiment, compared with control group, there was no significant change in-expression of β2-GP1, VEGF and TF in sham operation group(P〉0.05). Levels of β2-GP1, VEGF and TF were in-creased at the 2ndhour and 8thhour then peak at the 24 thhour which was higher than those in the 24 thhour control groupand in Sham group and it was also higher than those in the 2ndhour and the 8thhour in model group with statistical signifi-cant difference(P〈0.01). Conclusion Based on the above experimental data, in rat DVT formation process, β2-GP1,VEGF and TF may play an important role in promote DVT formation.
出处 《天津医药》 CAS 北大核心 2014年第12期1176-1179,共4页 Tianjin Medical Journal
基金 国家自然科学基金资助项目(81060151) 云南省卫生科技计划项目(2012ws0007) 云南省科技厅重点新产品开发计划项目(2010BC010)
关键词 β2糖蛋白Ⅰ 血管内皮生长因子类 凝血致活酶 静脉血栓形成 大鼠 Sprague-Dawley beta2-glycoprotein Ⅰ vascular endothelial growth factors thromboplastin venous thrombosis rats Sprague-Dawley
  • 相关文献

参考文献14

  • 1Tenna AM, Kappadath S, Stansby G. Diagnostic tests and strategies in venous thromboembolism[J]. Phlebology, 2012, 27(2): 43- 52. doi: 10.1258/phleb.2012.012S35.
  • 2Vazquez SR, Kahn SR. Advances in the diagnosis and management of postthrombotic syndrome[J]. Best Pract Res Clin Haematol, 2012,25(3): 391-402. doi: 10.1016/j.beha.2012.06.006.
  • 3Ashrani AA, Silverstein MD, Lahr BD, et al. Risk factors and under- lying mechanisms for venous stasis syndrome: a population-based case-control study[J]. Vascular Medicine, 2009, 14(4): 339-349. doi: 10.1177/1358863X09104222.
  • 4Kumar DR, Hanlin E, Glurich I, et al. Virchow' s contribution to the understanding of thrombosis and cellular biology[J]. Clin Med Res, 2010, 8(3-4): 168-172. doi: 10.3121/cmr.2009.866.
  • 5Kyrle PA, Eichinger S. Is virchow' s triad complete [J]? Blood, 2009, 114(6): 1138-1139. doi: 10.1182/blood-2009-05-223511.
  • 6Maekman N. Triggers, targets and treatments for thrombosis[J]. Na- ture, 2008, 451(7181): 914-918. doi: 10.1038/nature06797.
  • 7Persijn L, Deeavele AS, Sehouwers S, et al. Evaluation of a new set of automated assays for anticardiolipin and an- ti-beta2-glycoprotein I antibodies in the laboratory diagnosis of the antiphospholipid syndrome[J]. Thromb Res, 2011, 128(6):565- 569. doi: 10.1016/j.thromres.2011.04.004.
  • 8Mineo C, Shaul PW. New insights into the molecular basis of the an- tiphospholipid syndrome[J].Drug Discov Today Dis Mech, 2011, 8 (1):47-52.doi: 10.1016/j.ddmec.2011.12.002.
  • 9Waltham M, Evans CE, Humphries J, et al. Protein fragments from the VEGF binding domain of fibronectin are expressed in distinct spatial and temporal patterns during venous thrombus resolution[J]. Thromb Res, 2012, 130(2):281- 284. doi: 10.1016/j. thromres.2012.05.006.
  • 10Olszewska-Pazdrak BL, Hein TW, Olszewska P, et al. Chronic hy- poxia attenuates VEGF signaling and angiogenic responses by down- regulation of KDR in human endothelial cells[J]. Am J Physiol Cell Physiol, 2009, 296(5):C 1162- 1170. doi: 10.1152/ajp- ce11.00533.2008.

同被引文献35

  • 1邱贵兴,王炜.骨科领域静脉血栓栓塞症的预防[J].中国血管外科杂志(电子版),2011,3(1):9-11. 被引量:21
  • 2Rosendaal FR. Venous thrombosis: a muhicausal disease [ J ]. Lancet, 1999, 53(9159): 1167-1173.
  • 3Rosen SD, Bertozzi CR. The selectins and their ligands[ J]. Curr Opin Cell Biol, 1994, 6(5) : 663 -673.
  • 4Nomura S, Fumiko O, Misao A, et al. Platelets expressing P - selectin and platelet - derived microparticles in stored platelet concentrates bind to PSGL - 1 on filtrated leukocytes [ J ]. Clin Appl Thromb Hemost, 2000, 6(4): 213-221.
  • 5Diaz JA, Obi AT, Myers DD Jr, et al. Critical review of mouse models of venous thrombosis [ J ]. Arterioscler Thromb Vasc Biol, 2012, 32(3): 556-562.
  • 6科技部.中华人民共和国科技部关于善待实验动物的指导性意见[S].2006.
  • 7Fernandes LS, Conde ID, Wayne Smith C, et al. Platelet - monocyte complex formation: effect of blocking PSGL - 1 alone, and in combination with alphallbbeta3 and alphaMbeta2, in coronary stenting[ J ]. Thromb Res, 2003, 111 (3) : 171 - 177.
  • 8da Costa Martins P, van den Berk N, Ulfman LH, et al. Platclet-monocyte complexes support monocyte adhesion to endothelium by enhancing secondary tethering and cluster formation[ J 1. Arterioscler Thromb Vasc Biol, 2004, 24 ( 1 ) : 193 - 199.
  • 9Yokoyama S, Ikeda H, Haramaki N, et al. Platelet P - selectin plays an important role in arterial thrombogenesis by forming large stable platelet - leukocyte aggregates [ J ]. J Am Coll Cardiol, 2005, 45(8) : 1280 -1286.
  • 10Zhao Jm, HE ML, Xiao ZM, et al. Different types of intermittent pneumatic compression devices for preventing venous thromboembolism in patients after total hip replacement [ J ]. Cochrane Database Syst Rev, 2012, 11: CD009543.

引证文献3

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部