β2-GP1、VEGF、TF与大鼠DVT形成的关系

The Correlation between β2-GP1,VEGF and TF with Rat DVT Formation

目的建立大鼠下腔静脉狭窄法深静脉血栓（DVT）模型,检测大鼠血液中β-2糖蛋白1（β2-GP1）、血管内皮生长因子（VEGF）、组织因子（TF）表达变化,研究其与DVT形成的关系。方法 70只SD大鼠,随机分为对照组（10只）,假手术组（30只）,模型组（30只）,模型组于造模后2 h、8 h、24 h分为3个亚组,采集各组大鼠下腔静脉血5 m L,ELISA法检测β2-GP1、VEGF、TF水平。结果大鼠血液中β2-GP1、VEGF、TF表达在对照组与假手术组间比较无统计学差异（P〉0.05）,造模后2~24 h,β2-GP1、VEGF、TF在血液中的表达呈上升趋势,24 h时达峰值,高于对照组、假手术组、造模后2 h组和造模后8 h组（P〈0.01）,其余4组间比较差异无统计学意义（P〉0.05）。结论大鼠DVT形成过程中,β2-GP1、VEGF、TF可能起到促进血栓形成的作用。

Objective To build rat DVT inferior vena cava partial stasis（narrow） model, to detected the expressionof β2-GP1, VEGF and TF in rat blood, and to investigat the correlation between β2-GP1, VEGF and TF with DVT. Methods SD rats（n=70） are divided into control group（n = 10）, sham operation group（n = 30） and the model group（n = 30） ran-domly and DVT model was built by the inferior vena cava partial stasis（narrow） after 2 h, 8 h and 24 h respectively. In eachtime point, ten rats were taken in each group, inferior vena cava blood were collected while β2-GP1, VEGF and TF expres-sion were detected by ELISA. Results In rat experiment, compared with control group, there was no significant change in-expression of β2-GP1, VEGF and TF in sham operation group（P〉0.05）. Levels of β2-GP1, VEGF and TF were in-creased at the 2ndhour and 8thhour then peak at the 24 thhour which was higher than those in the 24 thhour control groupand in Sham group and it was also higher than those in the 2ndhour and the 8thhour in model group with statistical signifi-cant difference（P〈0.01）. Conclusion Based on the above experimental data, in rat DVT formation process, β2-GP1,VEGF and TF may play an important role in promote DVT formation.