江西南昌郊区松树花粉过敏原的分离与鉴定

Isolation and immunological characterization analysis of allergen in pine pollen

目的对江西南昌郊区松树花粉的变应原组分进行分离及免疫学鉴定。方法提取松树花粉粗提液,然后通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对松树花粉的蛋白质组分进行分离并测定其分子量,采用免疫印迹(Western-blotting)法鉴定其变应原成分。结果松树花粉粗提液有10余条蛋白带,其中分子量为12k D、26k D和100k D的蛋白可与松树花粉过敏性病人血清Ig E结合,其中26k D为主要变应原。结论对松树花粉变应原进行了初步的分离、鉴定,为进一步对松树花粉变态反应性疾病的临床诊断和治疗提供初步的实验基础。

Objective To isolate and characterize the allergenic components of pine pollen. Methods Pine pollen coarse ex-traction fluid was extracted, and the proteins of pine pollen extraction and their molecular were analyzed by SDS-PAGE. To char-acterize the allergenic components, pine pollen coarse extraction fluid was analyzed by western-blotting. Results The pine pollen coarse extraction fluid displayed more than ten protein bands by SDS-PAGE,and three bands （relative molecular weights were 12 kD, 26 kD and 100 kD, respectively） could react with IgE in the sera of patients with allergy to pine pollen. Among the three bands, the 26 kD protein was the major allergen. Conclusion Our study of preliminary isolation and immunological characteriza-tion of the allergenic components in pine pollen will be used as a base for diagnosis and therapy of pine pollen related allergy.