菊花品种神马的茎段离体快繁研究

Study on Stem Micropropogation of Chrysanthemum cv. Jinba

为加快优质菊花种苗的繁殖,以菊花品种神马的茎段为试材,建立了无菌操作体系,并进行继代增殖、生根培养和驯化移栽。结果表明:升汞消毒茎段5min效果最佳,存活率可达70.0%,污染率为13.30%;最佳初代培养基为MS+BA 1.0mg·L-1+NAA 0.1mg·L-1,萌发率高且生长良好;最佳增殖培养基为MS+BA1.0mg·L-1+NAA0.1mg·L-1,增殖系数达到6.2;最佳生根培养基为1/2MS+NAA0.1mg·L-1,生根率可达97.8%。

In order to speed up the breeding of high-quality Chrysanthemumcv.Jinba,taking stem segment of Chrysanthemumcultivar Jinba materials,sterile system was established,and the axillary bud in vitro was subcultured,rooted,domesticated and transplanted.The results showed that the disinfection time by 0.1% HgCl2 for 5minutes was optimal,and the survival rate could be up to 70.0%,and contamination rate was 13.3%.When the induction medium was MS＋BA 1.0mg·L-1＋NAA 0.1mg·L-1,the germination rate was high and the plantlets grew well.The optimal subculture medium was MS＋BA1.0mg·L-1＋NAA0.1mg·L-1 with multiplication coefficient of 6.2,and the most suitable rooting medium was 1/2MS＋NAA0.1mg·L-1,with rooting rate over 97.8%.