摘要
为建立玫瑰组织培养体系,研究以红花玫瑰嫩茎为初次诱导外植体,对红花玫瑰的离体培养途径进行研究。结果表明:芽诱导培养基配方为MS+6-BA 2.5 mg·L-1+NAA 0.3mg·L-1+蔗糖30g·L-1+琼脂6g·L-1;增殖培养配方为MS+6-BA 3.0mg·L-1+NAA 0.2mg·L-1+蔗糖30g·L-1+琼脂6g·L-1;生根培养配方为1/2 MS+NAA 0.2mg·L-1+蔗糖30g·L-1+琼脂6g·L-1。
In order to establish tissue culture system of rose,taking nodal segments as explants,the culture way of red rose was studied.The results showed that the optimum initial cultural medium was MS+6-BA2.5mg·L-1+NAA 0.3 mg·L-1+sucrose 30g·L-1+agar 6g·L-1;The optimum multiplication medium was MS+6-BA 3.0mg·L-1+NAA 0.2mg·L-1+sucrose 30g·L-1+6g·L-1 agar;The optimum root inducing cultural medium was 1/2 MS+NAA 0.2mg·L-1+sucrose 30g·L-1+agar 6g·L-1.
出处
《黑龙江农业科学》
2014年第12期23-24,共2页
Heilongjiang Agricultural Sciences
关键词
玫瑰
嫩茎
组织培养
芽诱导
增殖培养
生根培养
red rose
nodal segments
tissue culture
bud induction
multiplication culture
root culture