黑龙江省稻瘟病菌无毒基因的检测

Detection on Avirulence Gene of Rice Blast in Heilongjiang Province

为了探索黑龙江省稻瘟病菌无毒基因的分布情况,通过分子标记法对黑龙江省19个地区的204个稻瘟病菌的菌株进行PCR扩增,检测是否含有无毒基因Avr-pita、AVR-CO39、PWL2和PWL3。结果表明:各基因的出现频率差异较大,PWL2的出现频率较大,为52.94%,PWL3最低,出现频率为0,Avr-pita和AVRCO39的出现频率分别为39.22%和37.75%。黑龙江省稻瘟病菌的群体结构复杂,在不同地区甚至相同地区的菌株中无毒基因的存在情况都有明显的差异。

In order to explore the distribution of rice blast fungus avirulent genes in Heilongjiang province,204 strains of Magnaporthe grisea were amplified by PCR through molecular marker method in 19 regions of Heilongjiang province isolates,the presence of avirulence genes Avr-pita,AVR-CO39,PWL2 and PWL3was detected.The results showed that the gene frequency had larger difference,the frequency of PWL2 was 52.94%which was the highest,PWL3 was the lowest,the frequency was 0,Avr-pita and AVR-CO39 frequency were39.22% and 37.75%,respectively.The population structure of rice blast in Heilongjiang province in the presence was complex,avirulence genes in different regions and even in the same region strains had obvious differences.