摘要
利用柱层析色谱法对龙胆草提取物进行分离,研究洗脱剂、洗脱剂比例等因素对龙胆草分离效果的影响。结果表明:龙胆苦苷的最适洗脱剂为氯仿-甲醇体系,氯仿∶甲醇∶水的最佳比例为65∶35∶10。
The extraction of gentian was separated by column chromatographic.The effect of eluent and its ratio on separation effect was studied.The result showed that：the suitable eluent for extraction of gentiopicroside was chloroform-methanol system,the optimal ratio of chloroform,methanol and water was 65∶35∶10.
出处
《黑龙江农业科学》
2014年第12期133-135,共3页
Heilongjiang Agricultural Sciences
关键词
龙胆草
柱层析
分离
gentians
column chromatographic
separation
