精氨酸对孕鼠子宫NO和微血管的影响

Effects of Arginine on NO and Microvessels of Uteroplacenta in Mice

研究精氨酸(Arg)对胎鼠子宫微血管的影响,阐明其缓解宫内发育迟缓(IUGR)的部分机理。将120只雌性小鼠随机分为6组,即对照组,A组(基础日粮添加2.5g/kg Arg,下同),B组(5g/kg Arg),C组(7.5g/kg Arg),D组(10g/kg Arg),E组(15g/kg Arg)。试验期间各组自由采食和饮水。每组随机抽取10只孕鼠进行眼球摘除采血,颈椎脱臼处死孕鼠,分别检测IUGR、子宫胎盘的微血管密度、NOS活力和NO含量。结果与对照组相比,D组可显著降低IUGR发生率达64.99%(P<0.05)。B、C、D、E组可极显著(P<0.01)提高小鼠胎盘NOS活力及NO含量,各组子宫胎盘均未见病变。B组比对照组子宫微血管密度和胎盘微血管密度分别增加了16.30%和10.58%,但未达到显著水平(P>0.05)。说明Arg可能通过改善子宫和胎盘血液循环和母胎营养物质供应,达到缓解IUGR的作用。

The objective is to investigate the effects of arginine on fetal microvessels in mice,to illuminate the mechanismts of the intrauterine growth retardation （IUGR）.120 female mice were randomly divided into six groups according to arginine（Arg）levels supplemented in diets.Arg supplements in groups con-trol,A,B,C,D,E were 0,2.5,5,7.5,10,15 mL/kg,respectively.Mice consumed the diets and water free-ly.10 mice from each group were randomlyselected and killed,detecting IUGR,microvessel density of u-terus and placenta,distribution NOS activity and NO content on gestational 16th day.Compared with the control mice,the dietary supplementation with B to E Arg decreased the IUGR rates.The uterine NOS ac-tivity,placental NOS activity and placental tissue NO content of pregnancy mice in B,C,D,E were signif-icantly increased （P 〈0.01）,pathological changes wasn′t found in the experiment.The microvessel densi-ties of uterus and placenta in group B were increased by 16.30% and 10.58% respectively compared with control,but not significant （P 〉0.05）.The results indicated that Arg can improve uteroplacental circula-tion and maternal-fetal nutritive substance supplement,thus contributing to relieve IUGR.