摘要
核酸疫苗为科研人员在疾病预防控制方面开辟了新的途径,然而目前大规模制备既符合相关质量要求,又经济的重组质粒DNA,特别是药品级重组质粒DNA,仍是制约核酸疫苗和基因治疗研究的瓶颈。如何获得合格的药用质粒DNA并建立大规模生产平台成为一个重要的研究课题,论文综述了近年来核酸疫苗上游生产工艺的技术进展,以供参考。
Nucleic acid vaccine has opened up new avenues for researchers in terms of disease prevention and control currently however,large-scale preparation of plasmid DNA is still restricted bottlenecks in gene therapy research consistent with the relevant quality requirements and economical recombinant,especially biologicals grade.It is becoming an important research topic to get qualified medicinal plasmid DNA and to establish large-scale production platform.In this review,we briefly summarized recent advances of the up-stream production technology for DNA vaccine.
出处
《动物医学进展》
CSCD
北大核心
2014年第12期140-143,共4页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31201913)
山东省自然科学基金项目(ZR2012CQ012)
关键词
核酸疫苗
质粒
发酵
分批补料培养
nucleic acid vaccine
plasmid
fermentation
fed-batch culture
