肠道病毒71型感染对细胞内钙离子分布的影响

The effect of infection of enterovirus 71 on the distribution of cell Ca^(2+)

利用流式细胞技术和激光共聚焦显微镜技术观察EV71感染前后,细胞内钙离子分布变化情况。细胞感染EV71后,细胞内质网内钙离子浓度显著降低,细胞质及线粒体内钙离子浓度显著升高。EV71的感染能够引起宿主细胞内钙离子的分布变化,这种变化可能会调节病毒与宿主细胞间的一系列相互作用。

To investigate the distribution of the calcium（ Ca^2＋ ） in cells infected with enterovirus 71,flow cytometer and confocal microscope were used to detect Ca2＋ in endoplasmic reticulum（ ER）,mitochondria and cytoplasma labeled with specific dye. Results showed that EV71 infection resulted in the reduction of Ca^2＋ content in endoplasmic reticulum（ ER） whereas raised the cytosolic Ca^2＋ concentration and the infux of Ca^2＋ in mitochondria. It indicated that EV71 infection influenced the distribution of host intracellular Ca2＋ by releasing ER Ca^2＋ . The redistribution of intracellular Ca^2＋ will definitely effect normal cell homeostasis and cell functions.