超微粉中药对保育猪腹泻及肠黏膜免疫细胞的影响

Effect of Superfine Chinese Herbal Medicine on Diarrhea and Intestinal Mucosal Immune Cells in Nursery Pigs

为研制安全、高效环保型保育猪中药添加剂,将72头体重相近的28日龄杜长大断奶仔猪随机分为4组,每组3个重复,每个重复6头猪,分别饲喂基础饲粮、基础饲粮+抗生素、基础饲粮+1%中草药组方Ⅰ、基础饲粮+1%中草药组方Ⅱ,进行为期28 d的饲养试验,统计腹泻频率及腹泻指数、测定肠黏膜免疫细胞。结果显示,中药Ⅱ组效果优于中药Ⅰ组和抗生素组。中药Ⅱ组腹泻持续时间最短、腹泻频率及腹泻指数最低;与对照组相比,对回肠段杯状细胞数量影响最大的是抗生素组,提高14.29%(P>0.05)。对其它肠黏膜免疫细胞数量影响最大的是中药Ⅱ组,提高十二指肠、空肠及回肠段上皮内淋巴细胞数量分别达23.37%(P<0.01)、16.33%(P<0.01)、14.52%(P<0.01);提高十二指肠及空肠段杯状细胞数量分别达31.82%(P<0.01)、26.92%(P<0.01);提高十二指肠、空肠及回肠段肥大细胞数量分别达17.99%(P<0.01)、23.87%(P<0.01)、18.71%(P<0.01)。

For the development of a safe,efficient and environmentally friendly Chinese herb additive in weaned piglets,72 Duroc-Landrace-Yorkshire weaned piglets which were randomly divided into four groups,each treatment had 3 replicates and each consisting of 6 piglets.The control group was fed the basal diet.Antibiotic group was fed the basal diet plus antibiotics.Chinese herbsⅠgroup was added 1% Chinese herbs formulaⅠ,Chinese herbsⅡgroup was added 1% Chinese herbs formulaⅡ.Feeding trial lasted 28 days.Diarrhea frequency,diarrhea index and the number of immune cells in the intestinal mucosa were measured.The results were as followed：Chinese herbsⅡgroup are better than Chinese herbsⅠgroup and antibiotic group.Chinese herbsⅡgroup showed shortest duration of diarrhea,lowest diarrhea frequency and diarrhea index.Compared with the control group,the antibiotic group significantly affected the number of goblet cells of ileum,increased 14.29%（P〉0.05）.The study also showed Chinese herbsⅡgroup can significantly improve the number of the other immune cells in intestinal mucosa.The number of lymphocytes in the duodenum,jejunum and ileum was increased by 23.37%（P〈0.01）,16.33%（P〈0.01）, 14.52%（P〈0.01） respectively.The number of goblet cells in the duodenum and jejunum was increased by 31.82%（P〈0.01） and 26.92%（P〈0.01） respectively.The number of mast cells in the duodenum,jejunum and ileum was increased by 17.99%（P〈0.01）,23.87%（P〈0.01） and 18.71%（P〈0.01） respectively.