金华地区仔猪致病性大肠杆菌的血清型鉴定及药敏试验

Serotype identification and drug sensitivity test of pathogenic E. coli from the piglet in Jinhua

为了调查金华地区仔猪大肠杆菌的耐药性及流行血清型,从金华地区21个猪场采集96份仔猪大肠杆菌病病料进行病原菌的分离和生化鉴定,结果分离到大肠杆菌79株,其中致病性大肠杆菌68株,对68株致病性大肠杆菌进行了8种抗生素敏感性试验,发现对丁胺卡那霉素、氟苯尼考、头孢噻呋最敏感,庆大霉素、红霉素、环丙沙星等抗菌药虽然有一定效果,但大多是低敏感或中度敏感,而对氨苄青霉素、诺氟沙星则基本耐药。对分离的68株致病菌进行了血清型鉴定表明,共有O13,O48,O54,O98,O146,O149,O161等7种血清型,其中O13,O54,O98为优势血清型。

In order to investigate the antibiotic resistance and predominant serotypes of porcine enteropathogenic E. coli, 79 strains of E. coli had been isolated from 21 pig farms in Jinhua. All of them were identified with biochemical tests and abdominal injection to mice for characters of pathogenesis. As a result, 68 strains were proved to be pathogenic. Of all the isolates, 68 strains were tested with resistance to 8 kinds of antibiotics. As a result, the isolated E. coli stralus to amikacin, florfenicol, ceftiofur were the most sensitive; Gentamicin, erythromycin, ring although cip- rofloxacin and other antibacterial drugs had some effect, but were largely low-sensitive or moderately sensitive; to ampicillin, norfloxacin were basic resistance. 68 strains were serotyped, among which 7 kinds of serotypes and the predominant serotypes are O13 , O54, O98.