摘要
目的探讨兔VX2肝癌模型中肝动脉化疗栓塞后肝癌微环境中CD+3、CD+4T细胞的表达情况。方法 56只健康清洁级新西兰兔经包块埋植法制作成VX2兔肝癌模型成功2周后,给予肝动脉化疗栓塞+动脉注射中等剂量干扰素-α,术后1周处死动物,分别选取癌组织、癌旁组织及正常肝组织制成石蜡切片,采用免疫组化检测微环境中CD+3、CD+4T细胞的表达情况。结果 50只模型兔在术后1周成功获得标本,病理观察提示肿瘤边缘形成包膜,包膜内和肿瘤间质未见明显血管。免疫组化提示CD+3、CD+4T细胞在三种组织中的表达有显著性差异(P<0.001),其中以癌旁组织最高,其次是癌组织。结论 CD+3、CD+4T细胞通过改变肝癌免疫微环境,在调节肿瘤免疫方面发挥重要作用。
Objective To examine the expression of CD3^＋ and CD4^＋ T cells in the mieroenvironment resulting from transhepatie arterial ehemoembolization（TACE） as per the rabbit VX2 hepatocareinoma model. Method 56 healthy New Zealand rabbits were enrolled by implanting the tumor fragment into the left liver and then rabbit VX2 hepatocareinoma model was established. 2 weeks later, all of the models were treated with TACE and injection of Interferon-odn moderate doses through artery. The experimental rabbits were sacrificed 1 week after surgery. The hepatoma tissue, paraeancerous tissue and normal liver tissue were respectively selected and made into paraffins which were then detected by immunohistochemistry to observe the expression of CD3^＋ and CD4^＋ T cells in the microenvironment. Findings Specimens were gained from 50 rabbit models 1 week after TACE. Pathological observation indieated that envelopes formed in the tumor margin, but inside the envelope and tumor stroma, obvious blood vessels were nearly not recognized. Imunohistoehemistry expression of CD; and CD4^＋ T cells among three tissues showed significant differences（ P 〈 0. 001 ）.Paracancerous tissue was the highest, followed by hepatoma tissue. Normal liver tissue was the lowest. Conclusion The higher expression of CD3^＋ and CD4^＋ T ceils in the microenvironment of rabbit VX2 hepatoeellular carcinoma model after TACE and injection of Interferon-α showed that CD3^＋ and CD4^＋ T cells played an important role in adjusting tumor immunity by changing the immune mieroenvironment of hepatocarcinoma.
出处
《健康研究》
CAS
2014年第6期622-624,共3页
Health Research
基金
浙江省卫生厅一般项目A类(2010KYA191)
