紫外分光光度法测定香菊胶囊黄酮总含量

Determination of Total Flavonoids in Xiangju Capsule by Ultraviolet Spectrometry

目的：建立香菊胶囊中黄酮总含量的测定方法。方法：采用紫外分光光度法，以芦丁为对照品，检测波长525nm，以线性、精密度、重现性、稳定性、回收率等作为效能指标，进行方法学验证。结果：回归方程为Y=0．476OX-0．0037，r=0．9998，化香树果序黄酮总含量在0．2012～1．0030mg范围内线性关系良好，平均加样回收率为98．2％，RSD-1．83％。结论：该方法灵敏快速，准确可靠，可用于香菊胶囊中黄酮成分总合量的测定。

Objective：To establish the determination method for total flavonoids from Xiangju Capsule. Methods：The contents of total flavonoids were determined by UV spectrophotometry at detection wavelength of 525 nm with rutin as standard substance, u- sing linear ity and linear range, accuracy, reproducibility, selectivity as the performance parameters. Results：The linear regression e quation was Y：0. 476 0X-0. 003 7（r：0. 999 8）. The linear range of rutin were 0. 201 2～1. 003 0rag with average recovery of 98.2% （RSD= 1.83 %, n= 6）. Conclusion：This method is sensitive, accurate and rapid, which can be used for quality control of to- tal flavonoids in Xiangju Capsule.