不同治疗方法对子宫腺肌病模型小鼠血管生成相关因子的影响

The Study of Influences on Angiogenesis Correlator of Adenomyosis Mice Model by Different Treatment

目的:探讨在子宫腺疾病(AM)在位及异位子宫内膜不同空间部位,中西医治疗方法对模型小鼠血管生成相关因子的不同影响。方法:对8周龄ICR雌鼠施行雄鼠垂体宫腔内移植手术,术后分别饲养3个月,形成模型组。选择导师行气化瘀法临床验方(蒲灵化瘀止痛方)为实验药物(A组),同时将丹莪妇康煎膏作为中药对照组(B组)、孕三烯酮组为西药对照组(C组)、未造模同期小鼠为空白组,各组分别给药3个月。运用免疫组化技术观察血管生成相关因子(VEGF、CD31、MMP-2)在模型组、用药组的变化。结果:与正常组比较,模型组在位内膜CD31表达显著下降(P<0.05),A、B、C组治疗后上调,且A组显著优于B、C组(P<0.05)。与模型组比较,A、B、C组异位内膜CD31表达显著下降(P<0.05),A、B、C组之间比较差异无统计学意义(P>0.05)。与正常组比较,模型组在位内膜MMP-2、VEGF显著升高(P<0.05)。治疗后A、B、C组在位内膜MMP-2、VEGF表达与模型组比较均显著下调(P<0.05),A、B、C组之间比较差异无统计学意义(P>0.05)。与模型组比较,治疗后A、B、C组异位内膜MMP-2均显著下降(P<0.05),C组显著低于A组及B组(P<0.05)。结论:蒲灵化瘀止痛方可以有效改善在位内膜的缺血缺氧状态,通过终止疾病的始动环节而达到治疗该病的目的。在改善局部血供方面,行气活血中药与西药比较有明显优势。

Objective：To observe the express of angiogenesis correlator, investigate managed mechanism of different method in the different position of the endomembrane.Method：The ICR mouse model was built by pituitary intrauterine transplant operation. Clinical effective medical prescription of mentors（PLF） was selected as the experimental drug（the group A）. At the same time, Danefukang decoction paste was selected as the Chinese medicine control group（the group B）, the Gestrinone group as the western medicine control group（the group C）, the mice in the same period of unwrought mode to the blank group, each group respectively for 3 months. The angiogenesis related factor（VEGF and CD31, MMP-2） of the change of the model group, the medication group were observed by immunohistochemical technique.Result：Compared with the normal group, the in-ectopic CD31 expression of the model group was decreased significantly（P〈0.05）, after treatment the group A, B, C was increased, and the group A was significantly better than the group B and C（P〈0.05）. Compared with model group, ectopic endometrium CD31 expression of the group A, B, C was decreased significantly（P〈0.05）, the differences between the group A and B, C had no statistical significance（P〉0.05）. Compared with the normal group, in-ectopic MMP-2, VEGF of the model group were significantly increased（P〈0.05）. The in-ectopic MMP-2, VEGF of the A, B, C groups expression after treatment were significantly lower than the model group（P〈0.05）, the differences between the group A and B, C had no statistical significance（P〉0.05）. Compared with the model group, ectopic endometrium MMP–2 of the group A and B, C after treatment were significantly decreased（P〈0.05）, the group C was significantly lower than that of the group A and B（P〈0.05）.Conclusion：PLF is correspond with the action of improvement of ischemia, anoxemia, in terms of improving local blood supply, the traditional Chinese medicine compare with western medicine has obvious advantages.