放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法

The method of establishing the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer

目的 探讨适用于放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法.方法 将人卵巢癌细胞株SKOV3分为常氧对照组、二氯化钴组和环境乏氧组,各组均在X射线单次照射（0、2、4、6、8 Gy）后72 h,应用噻唑蓝法检测细胞增殖.结果 与常氧对照组未照射细胞相比,二氯化钴组和环境乏氧组未照射细胞的细胞增殖率均显著降低（t=24.789、196.960,P均＜0.01）;与同组未照射细胞相比,常氧对照组与二氯化钴组接受不同剂量的X射线单次照射后,其细胞存活率均显著性下降（F=2263.039、3672.044,P均＜0.01）,且放射剂量越大,其细胞存活率越低,而环境乏氧组无显著性变化（F=1.412,P＞0.05）;与常氧对照组、二氯化钴组的同剂量照射细胞相比,环境乏氧组细胞存活率均显著升高（2Gy：F=61.125; 4Gy：F=181.825;6Gy：F=373.830; 8Gy：F=2425.510,P均＜0.01）.结论 环境乏氧组的细胞对射线产生了强烈的抵抗性,即放射敏感性显著性降低,射线对其杀伤力减弱,且所获得的乏氧细胞模型明显优于二氯化钴组,因此环境乏氧比二氯化钴更适合作为放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法.

Objective To investigate the suitable method of establishing the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer.Methods Human ovarian cancer cell line SKOV3 were divided into three groups：normoxic control group,cobalt chloride group and environmental hypoxia group.Cell proliferation of each group 72 h after single X-ray irradiation（0,2,4,6,8 Gy）were detected by MTT assay.Results Cell proliferation of non-irradiated cells in cobalt chloride group and environmental hypoxia group were significantly lower than that in normoxic control group （t =24.789,196.960,both P＜0.01）.Cell viability of the cells in normoxic control group and cobalt chloride group were significantly decreased after receiving a single X-ray irradiation of different doses compared with non-irradiated cells in the same group（F=2263.039,3672.044,bothP＜0.01） and their cell viability were decreased with the increase of radiation dose,while environmental hypoxia group had no significant change （F=1.412,P＞0.05）.The cell viability of irradiated cells in environmental hypoxia group were significantly higher than the irradiated cells with the same radiation in normoxic control group and cobalt chloride group（2 Gy：F=61.125; 4 Gy：F=181.825; 6 Gy：F=373.830; 8 Gy：F=2425.510,all P＜0.01）.Conclusions Cells in environmental hypoxia group were very resistant to radiation.Their radiation sensitivity strikingly decreased and the killing effect of radiation on them was weak.The hypoxic cellular model of environmental hypoxia group was obviously superior to cobalt chloride group.Environmental hypoxia method was more suitable to establish the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer compared with cobalt chloride.