旋毛虫ES抗原对DC2.4细胞TLR4、NF-κB和AP-1 mRNA表达的影响

Effect of ES antigen of Trichinella spiralis on expression of mRNA of TLR4,NF-κB and AP-1 in DC 2.4

目的探讨旋毛虫ES抗原对小鼠DC2.4细胞TLR4、NF-κB和AP-1表达的影响。方法以实时荧光定量PCR(Real-time FQ-PCR)技术对空白对照组、LPS组、ES抗原组、ES抗原+LPS/ES抗原组和LPS+LPS/ES抗原组,检测TLR4、NF-κB和AP-1基因mRNA表达水平变化。结果 ES抗原和LPS单作用组的TLR4、NF-κB和AP-1mRNA相对表达量分别是6.14/4.09/3.63和4.68/4.71/4.11,是ES抗原和LPS预作用组的各基因相对表达量的2倍以上,差异有统计学意义(P<0.05)。结论实验证实,DC2.4细胞受ES抗原和LPS持续作用或者双重作用均可诱导小鼠DC2.4细胞TLR4、NF-κB和AP-1表达的降低,可能诱致DC2.4细胞免疫耐受。

To explore the influence of Trichinella spiralis ES antigen on the expression of mRNA of TLR4,NF-κB and AP-1 in DC 2.4,we set up the following groups：blank control group,LPS group,ES antigen group,ES antigen＋LPS/ES antigen group,and LPS＋LPS/ES antigen group,which were presented by expression level of TLR4,NF-κB and AP-1 gene mRNA,and detected by fluorescence quantitative PCR （Real-time FQ-PCR） technology.Results showed that expression level of TLR4,NF-κB and AP-1 mRNA in pre-treatment of ES antigen and LPS group were significantly lower than that of ES and LPS group （P＜0.05）,respectively.We confirmed that the expression of mRNA of TLR4,NF-κB and AP-1 of DC2.4 could be suppressed by constant stimulation or combined effect of ES antigen and LPS.