二硫化碳吸入染毒对ApoE基因敲除小鼠和C57BL/6J小鼠血脂水平的影响

Effects of carbon disulfide inhalation on lipid levels of ApoE gene knockout mice and C57BL/6J mice

目的 探讨二硫化碳（CS2）吸入染毒对ApoE基因敲除小鼠和C57BL/6J小鼠血脂水平的影响.方法 将51只雄性ApoE基因敲除小鼠随机分为CS2染毒正常饮食组、CS2未染毒正常饮食组、CS2染毒高脂饮食组、CS2未染毒高脂饮食组.将50只C57BL/6J雄性小鼠也按同样的方式分成4组.将CS2染毒组,以浓度为1 000 mg/m3的CS2进行静式吸入染毒实验,5 h/d,5d/周,共4周.每周称量并记录每只小鼠的体重1次.染毒第14天,每组随机抽取6只小鼠,测量每只小鼠血清中胆固醇（TC）的水平.染毒第28天,测量剩余小鼠血清中胆固醇（TC）和低密度脂蛋白（LDL）水平.结果 CS2染毒组小鼠的平均体重增加均小于未染毒组.染毒第14、28天,ApoE基因敲除组小鼠染毒高脂饮食的TC水平高于ApoE基因敲除未染毒高脂饮食组,差异有统计学意义（P值均＜0.01）.染毒第14天,C57BL/6J小鼠未染毒高脂饮食组TC水平高于C57BL/6J小鼠未染毒正常饮食组,差异有统计学意义（P＜0.05）.染毒第28天,ApoE基因敲除染毒高脂饮食组小鼠的LDL水平高于ApoE基因敲除未染毒高脂饮食组,差异有统计学意义（P值=0.003）.结论 CS2染毒、ApoE基因敲除和高脂饮食均有促进小鼠血脂升高的作用,增加了动脉粥样硬化的风险.

Objective To investigate the effects of carbon disulfide （CS2） inhalation on the lipid levels of ApoE knockout gene mice and C57BL/6J mice.Methods Fifty-one male ApoE gene knockout mice were randomly divided into four groups：CS2-exposed normal diet group,CS2-unexposed normal diet group,CS2-exposed high-fat diet group,and CS2-unexposed high-fat diet group.Fifty male C57BL/6J mice were divided into four groups in the same way.The exposed groups received 1000 mg/m3 CS2 by static inhalation （5 h/d,5d/w） for four weeks.The weight of each mouse was determined and recorded once a week.On the 14th day of exposure,six mice in each group were randomly selected to measure serum total cholesterol （TC） levels.On the 28th day of exposure,the serum levels of TC and low-density lipoprotein （LDL） in the remaining mice were measured.Results The mean weight gain of exposed groups was less than that of the unexposed groups.On the 14th and 28th days of experiment,the TC levels of the CS2-exposed high-fat diet group were significantly higher than those of the CS2-unexposed high-fat diet group among ApoE knockout gene mice （P ＜0.01 for both）.On the 14th day of experiment,the TC levels of the CS2-unexposed high-fat diet group were significantly higher than those of the CS2-unexposed normal-diet group among C57BL/6J mice group （P＜0.05）.On the 28th day of experiment,the LDL levels of the CS2-exposed high-fat diet group were significantly higher than those of the CS2-unexposed high-fat diet group among ApoE knockout gene mice （P=0.003）.Conclusion CS2 exposure,high-fat diet,and ApoE gene knockout can elevate blood lipids in mice,thus increasing the risk of atherosclerosis.